Infinite® 200 - Download

The Infinite® 200 NanoQuant is the first multimode microplate reader to be developed specifically for absorbance applications with low sample volumes. The sensitive new instrument is available with Tecan’s quad4 monochromators™ or with UV-stable filters, and can detect DNA concentrations as low as 1 ng/μl. This affordable new instrument represents a simplified version of the standard Infinite 200 and is compatible with Tecan’s new NanoQuant Plate™.

The Infinite® 200 series offers an affordable and easily upgraded line-up of flexible and scalable microplate readers, incorporating all maindetection methods and for all applications. The unique modular design and adaptability of the Infinite 200 series provides a choice of an advanced quad4 monochromator system or a filter system, and you can tailor the instrument’s detection modes to suit your requirements. The Infinite can be integrated with Tecan’s Freedom EVO® liquid handling workstations.

In this application note we describe the use of Tecan’s HydroFlexTM washer equipped with a magnetic bead plate carrier for fast purification of a large number of samples using magnetic beads.For the detection of the luminescence signal Tecan’s Infinite® F200 multimode reader was used.

With its NanoQuant Plate™, Tecan provides a new tool for reproducible and sensitive quantification as well as purity check of nucleic acids.
For quality control of oligonucleotide labeling reactions in real-time PCR assays and in array hybridization experiments using dye-labeled probes, the labeling efficiency is an impor-tant parameter to evaluate results. The NanoQuant Plate can be easily used to determine the labeling efficiency of nucleic acid probes.

Protein quantification is often required before proceeding with protein samples for isolation, chromatographic or electro-phoretic analysis, or immunohistochemical methods. Two different techniques are generally used for colorimetric detection and quantification of proteins: protein-dye binding and protein-copper chelation. In this note we describe the use of Tecan’s Infinite® F200 and Infinite M200 instruments for easy and sensitive protein quantification using different absorbance-based assays.

For protein assays in general, and especially for the modified Lowry protein assay, the reader-injector system is an ideal solution avoiding pipetting and workflow errors, and resulting in consistent and traceable data.Tecan´s Infinite® 200 instrument series extended with the injector system offers a multi-functional system, from injection of reagents, to mixing, incubation and measuring, with all steps performed within one instrument set-up.