Freedom EVO^® Genotyping - Download

Freedom EVO® Genotyping

Automated platform for SNP genotyping and analysis

The Freedom EVO Genotyping system provides a scalable and integrated set-up for automated genotyping of single nucleotide polymorphisms (SNPs). Being able to quickly and efficiently screen genomic DNA samples for phenotypically relevant SNPs has become increasingly important to a wide range of disciplines, including pharmacogenomics, population genetics and forensic medicine. The workstation can be configured to meet your requirements, offering rapid, reliable performance.

It reliably and efficiently performs many essential genotyping application steps, such as:

• DNA isolation and quantitation
• PCR / sequencing reaction set-up
• microplate sealing
• amplification/sequencing (using integrated, third-party thermal cyclers)
• PCR / sequencing clean-up
• assay detection.

ULTRA 384 Multi-Detection Microplate Reader Economical, high-performance reading of all plate formats up to 384-well microplates

The ULTRA 384 from Tecan is ideal for genomics, proteomics and drug discovery applications. It offers a cost-effective, high-performance solution for reading all types of microplate formats from 96-well to 384-well at a price tag affordable to academic, biotech and government labs. The ULTRA 384 combines four fluorescence detection modes—plus absorbance and glow luminescence - with high sensitivity and processing speed for reliable, medium throughput in a diverse range of applications. Also, a variety of...

Detection of a SNP in the Adenosine A2a receptor gene (ADORA2A) using fluorescence polarization of templatedirected

dye-terminator incorporation (FP-TDI)

Except for identical twins or clones, no two organisms are genetically identical. There is a large range of genetic variation between two members of a species. In humans, two unrelated genomes are estimated to vary between 1 in 500 - 1000 base pairs. Given an estimated size of the human genome of 3 x 109, then there are approximately 3 x 106 DNA sequences that are non-identical between two unrelated individuals. Most of the variations occur as Single Nucleotide Polymorphisms (SNPs). These variants are the substitution of a single base in the DNA sequence for another, such as a C being replaced with a T (or U). SNs are hypothesized to account for much of the genetic variation between individuals and are thought to affect processing of pharmacological agents and certain SNPs may confer higher risk of developing complex genetic diseases, such as, cancer, hypertension, diabetes etc. Although SNPs are numerous, genotyping of this form of genetic polymorphism has been difficult in many cases. One recently described method of enotyping SNPs, FP-TDI (Fluorescence Polarization Template directed Dye Incorporation) is...

Detection of a SNP in Adenosine A2a receptor gene using fluorescence polarization of

template-directed dye-terminator incorporation (FP-TDI)

Except for identical twins or clones, no two organisms are genetically identical. There is a large range of genetic variation between two members of a species. In humans, two unrelated genomes are estimated to vary between 1 in 500 - 1000 base pairs. Given an estimated size of the human genome of 3 x 109, there are approximately 3 x 106 DNA sequences that are non-identical between two unrelated individuals. Most of the variations of all known polymorphisms occur as Single Nucleotide Polymorphisms (SNPs). These variants are defined as substitution of a single base in the DNA sequence for another, such as a C being replaced with a T (or U) in case of Adenosine A2a receptor gene (ADORA2a). SNPs are hypothesized to account for much of the genetic variation between individuals and are thought to affect processing of pharmacological agents and certain SNPs may confer higher risk of developing complex genetic diseases, such as cancer, hypertension, diabetes etc. Although SNPs are numerous, genotyping of this form of genetic polymorphism has been difficult in many cases. One recently described method of genotyping SNPs, FP-TDI (Fluorescence Polarization Template directed Dye Incorporation) is simple, non-radioactive and relatively inexpensive. The method is...

TILLING in Dresden: large-scale detection of single nucleotide polymorphisms

TILLING (Targeting Induced Local Lesion IN Genomes), a powerful reverse genetics tool,was developed to identify single nucleotide polymorphisms (SNP) in mutagenized individuals by PCR amplification of a gene of interest from each individual, followed by SNP detection1,2. However, because the identification of nonsense mutations leading to loss of gene function is a relatively rare event, it is necessary to screen thousands of mutagenized individuals to find a presumptive null mutation in a gene of interest. TILLING is mainly based on PCR followed by enzymatic digests or direct sequencing of PCR-fragments, and...

The importance of high throughput SNP genotyping for complex disease research in Spain

The technique of SNP analysis has become more widely used over the last four to five years because the high density maps it provides make it easier to locate small but potentially significant changes in the genome. It is useful for a wide range of applications – from biomedical investigations in hospitals and academic institutes to drug development studies for biotech or pharmaceutical companies. Three laboratories in Barcelona, Madrid and Santiago de Compostela, collectively the Centro Nacional de Genotipado (National Genotyping Centre)(CeGen), were established in 2003/4 to provide a flexible and...