Laser Scanner - Specifications
General
- Possible Laser Sources 635 nm, 532 nm, 488 nm, 594 nm
- Detectors 1 or 2 PMTs, optional simultaneous dual color detection
- Emission Filters 1 or 2 filter slides, space for 4 filters each
- 1 Laser System: CY5 (692/45),
- 1-4 Laser System: CY5, ROX (635/35), CY3 (575/50), FITC (535/25) standard filters
- Additional filters can easily be added. Up to 20 filters can be applied per system and registered by LS Software.
- Substrates as microscope slides, microplates or any other substrate up to the size of microplates (15x85x127 mm)
- Scan area slides 22x75 mm one run; 1-96 scan areas over an area up to microplate footprint (automated stitching)
- Autofocus for transparent or not-transparent surfaces with or without segmentations performed in advance to each individual scan
- Depth of Focus 90, 300, or 800 µm reading by 3 software selectable pinhole sizes
- Working Distance 6.5 mm (focus)
- NA 0.6
- Adjustable angle of incidence of laser beam 25-0° for evanescence resonance scanning and three-dimensional structures
- Interface to PC serial and high-speed data acquisition connection
- Power: 100 - 120 / 220 - 240 V, 50 / 60 Hz, 250 VA
- Dimensions: 813 mm (W) x 572 mm (D) x 370 mm (H)
- Weight 60 kg
Performance
- Signal to electronic noise ratio 5 orders of magnitude
- Sensitivity < 0.1 Fluorophore equivalent/µm²
- <0.01 Fluorophore equivalent//µm² by adjusted laser beam angle to Cy3/Cy5 evanescence resonance slide scanning
- Intrascenic Dynamic Range 4.5 orders of magnitude
- Gain Adjustment 5 orders of magnitude (0.005% to 500%)
- Pixel Resolution 6, 10, 20, 40 µm
- Reading speed 4 minutes for a full slide, 25 minutes a SBS plate (dual color, 10 µm pixel resolution),
PC Dell (minimum configuration delivered with instrument)
Pentium® IV 2,6 GHz, 2 GByte RAM, 80 GByte Hard Disk, CD-ROM /DVD Writer, Network card, Analog Modem, ArrayPro USB protection key (single site or network), 17" Color TFT Monitor, Microsoft® Windows® 2000, Office Software package, PCanywhere, WinZip
Options:
- Barcode reader for top-reading microscope slides, or
- Barcode reader for bottom-reading microscope slides (inverse scanning)
- Additional slide holder for 4 (25x75x1 mm) slides
- LaserCheck™ quality control tool kit (SBS format)
- Gel-adapter with lid e.g. for (72x108x5 mm) gels
- Adapter for larger (75x116x1 mm) glass plates
- Additional filter slide carrier for 4 emission filters
- Additional fluorescence (e.g.520/10 nm) and absorbance filter from huge filter stock
Technical details are subject to change without notice. For detailed information please contact your local Tecan sales organisation.
