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This poster describes the early implementation of BellBrook Labs’ new Transcreener® ADP2 Fluorescence Intensity (FI) assay on Tecan’s Infinite M200 monochromator-based
multimodular detection platform. The data presented on this poster clearly demonstrate the compatibility of the Infinite M200 with the new fluorescence intensity-based Transcreener technology. Optimized instrument settings to guarantee a maximized assay performance are stated.

Gene reporter assays are commonly used for the study of genetic regulatory promoter and enhancer or attenuator sequences. Using Invitrogen’s GeneBLAzer® technology the sequence of interest is cloned in front of a mammalian optimized bla-gene. Expression of the β-Lactamase gene results in enzyme activity cleaving a cell permeable FRET substrate containing two fluoroprobes, coumarin and fluorescein.
This poster describes the easy set-up and instrument settings of Tecan’s multimode reader Infinite F200 for bottom-mode FRET detection of the GeneBLAzer® technology.

Immediate access to all wavelengths in an affordable, scaleable detection family –with the patented NanoQuant Plate™ and Gas Control Module (GCM™)

• General questions
• Measuring with the NanoQuant Plate
• Software and technical questions
• Cleaning and maintenance
• Specifications and typical performance values

Fluorescence-based drug sensitivity testing using 3D tumor microtissues and the Infinite® M200 PRO monochromator-based multimode reader

Cell-based applications are central to life science research. They may range from cytotoxicity, proliferation, apoptosis and GPCR signaling assays to high-throughput screening (HTS) drug discovery applications. Adherent cell types are typically analyzed through the well bottom in order to bring the cell monolayer as close as possible to the detector and avoid unspecific fluorescence noise by the overlying growth medium. For this reason it is essential to guarantee illumination and reading of the entire well bottom, since cells are not always homogeneously distributed over the growth surface.

In vivo kinetic studies of drug uptake across the gastro-intestinal tract (GIT) and blood-brain barrier (BBB) are valuable tools for assessing bioavailability to prospective targets. These are relatively expensive and time consuming assays which are conducted sparingly. pION Inc. has introduced a parallel artificial membrane permeability assay (PAMPA) which has recently gained popularity as a novel, cost-effective high-throughput assay capable of rapidly screening compounds for their permeability characteristics in early drug discovery. In this note we describe the easy implementation of Tecan´s Infinite M200 with its absorbance scanning feature for PAMPA sample analysis.

In this application note we describe the use of Tecan’s HydroFlexTM washer equipped with a magnetic bead plate carrier for fast purification of a large number of samples using magnetic beads.For the detection of the luminescence signal Tecan’s Infinite® F200 multimode reader was used.

With its NanoQuant Plate™, Tecan provides a new tool for reproducible and sensitive quantification as well as purity check of nucleic acids.
For quality control of oligonucleotide labeling reactions in real-time PCR assays and in array hybridization experiments using dye-labeled probes, the labeling efficiency is an impor-tant parameter to evaluate results. The NanoQuant Plate can be easily used to determine the labeling efficiency of nucleic acid probes.

For protein assays in general, and especially for the modified Lowry protein assay, the reader-injector system is an ideal solution avoiding pipetting and workflow errors, and resulting in consistent and traceable data.Tecan´s Infinite® 200 instrument series extended with the injector system offers a multi-functional system, from injection of reagents, to mixing, incubation and measuring, with all steps performed within one instrument set-up.

 Determination of instrument detection limits using the Glowell™ GLO-466 standard

Enhanced Assay Performance using the Tecan Infinite® M200 PRO Multimode Reader

Enhanced performance using the Tecan Infinite® M200 multimode reader

Enhanced performance using the Tecan Infinite® M200 multimode reader

Optimizing the FI bottom sensitivity in the Infinite 200 PRO multimode reader series

Optimizing the luminescence sensitivity in the Infinite 200 PRO multimode reader series

Based on the successful Infinite 200 series, Tecan has developed the Infinite 200 PRO, with new enhancements, like the automated adjustable z-focusing in the Infinite M200 PRO. The adjustable z-focus for FI top measurements, which offers equally high sensitivity for all plate formats, helping to implement assay miniaturization. It is a flexible tool to customize measurement parameters to plate type and assay volume Z-focusing can be performed either by using the automated zcalculation function in the fluorescence intensity measurement stripe or by selecting the z-position option of the instrument menu in i-controlTM software. The z-position dialog of the instrument menu of Infinite M200 PRO provides an additional function which allows performing z-position scans for both a signal and a blank well for each fluorescent top measurement label. Beside the optimal z-position of signal and blank, the zposition for the maximum signal to blank ratio is also displayed. This automated z-adjustment with integrated background value correction is particularly suited to cell-based applications using autofluorescent growth media, providing automatic optimization of the signal-to-background ratio. The desired z-positions can be selected and applied to the measurement script by the user. In this technical note the impact of z-focusing on the sensitivity of fluorescence top measurements on Infinite M200 PRO is presented.

This Technical Note describes the successful
implementation of Invitrogen’s LanthaScreen TR-FRET assay system on Tecan’s Infinite F200 filter-based multi modular detection system. The Infinite F200 has performed according to Invitrogen’s LanthaScreen certification program criteria and was successfully validated by Invitrogen as “LanthaScreen® Certified”.

Scientists from Affymetrix® Inc., a world-leading supplier of microarray equipment and assays, evaluated the Tecan Infinite® 200 NanoQuant multimode microplate reader and two other spectrophotometers for DNA concentration analysis.
In this note, data are presented from a comparison done by Affymetrix using Tecan’s Infinite 200 NanoQuant, Molecular Devices SpectraMax® Plus and the Nanodrop® ND1000 instrument.

The Microbial Ecology and Host-Pathogen Interaction Laboratory (EMIP), of the INRA Institute in Montpellier, France, has already made great progress in research on virulence factors of insect pathogens.We have chosen the Infinite® M200 for our work because it is able to maintain shaking cultures over long periods and because various parameters, such as the temperature, agitation and the frequency of measurements, can all be programmed very easily using Tecan’s Magellan™ software.