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This Poster describes the successful implementation of Invitrogen’s LanthaScreen®
TR-FRET assay system on Tecan’s Infinite F200 filter-based multi modular detection
system. The Infinite F200 has performed according to Invitrogen’s LanthaScreen®
certifi cation program criteria and was successfully validated by Invitrogen as
“LanthaScreen® Certified”.

Transdermal drug delivery has extensively grown in the past decades. Advantages of transdermal application dosage forms like plasters in certain drug families have been proved. Efforts in drug research have been devoted to find a useful model for predicting the skin penetration of new molecules. The parallel artificial membrane permeability assay (PAMPA)1 as a passive permeation screen method can be used for the ADME screening of molecules.

Our purpose is to develop an effective skin PAMPA model using similar ingredients are present in the real human skin.

The Infinite 200 PRO NanoQuant was the first multimode microplate reader to be developed specifically for absorbance applications with low sample volumes. The sensitive instrument is available with Tecan’s Quad4 Monochromators™ or with UV-stable filters, and can detect DNA concentrations as low as 1 ng/μl. This affordable instrument represents a simplified version of the standard Infinite 200 PRO and is compatible with Tecan’s patent pending NanoQuant Plate™. The Infinite 200 PRO Nanoquant is the ideal solution for a broad range of applications, such as DNA- or RNA-quantification, quality control and labeling efficiency. It delivers excellent sensitivity, multiplexing capability and high format flexibility, including 6- to 384-well microplates as well as half-well plates and the NanoQuant Plate for low concentration and low sample volumes.

Cell-based applications are central to life science research. They may range from cytotoxicity, proliferation, apoptosis and GPCR signaling assays to high-throughput screening (HTS) drug discovery applications. Adherent cell types are typically analyzed through the well bottom in order to bring the cell monolayer as close as possible to the detector and avoid unspecific fluorescence noise by the overlying growth medium. For this reason it is essential to guarantee illumination and reading of the entire well bottom, since cells are not always homogeneously distributed over the growth surface.

In vivo kinetic studies of drug uptake across the gastro-intestinal tract (GIT) and blood-brain barrier (BBB) are valuable tools for assessing bioavailability to prospective targets. These are relatively expensive and time consuming assays which are conducted sparingly. pION Inc. has introduced a parallel artificial membrane permeability assay (PAMPA) which has recently gained popularity as a novel, cost-effective high-throughput assay capable of rapidly screening compounds for their permeability characteristics in early drug discovery. In this note we describe the easy implementation of Tecan´s Infinite M200 with its absorbance scanning feature for PAMPA sample analysis.

In this application note we describe the use of Tecan’s HydroFlexTM washer equipped with a magnetic bead plate carrier for fast purification of a large number of samples using magnetic beads.For the detection of the luminescence signal Tecan’s Infinite® F200 multimode reader was used.

With its NanoQuant Plate™, Tecan provides a new tool for reproducible and sensitive quantification as well as purity check of nucleic acids.
For quality control of oligonucleotide labeling reactions in real-time PCR assays and in array hybridization experiments using dye-labeled probes, the labeling efficiency is an impor-tant parameter to evaluate results. The NanoQuant Plate can be easily used to determine the labeling efficiency of nucleic acid probes.

For protein assays in general, and especially for the modified Lowry protein assay, the reader-injector system is an ideal solution avoiding pipetting and workflow errors, and resulting in consistent and traceable data.Tecan´s Infinite® 200 instrument series extended with the injector system offers a multi-functional system, from injection of reagents, to mixing, incubation and measuring, with all steps performed within one instrument set-up.

Enhanced Assay Performance using the Tecan Infinite® M200 PRO Multimode Reader

Enhanced performance using the Tecan Infinite® M200 multimode reader

Enhanced performance using the Tecan Infinite® M200 multimode reader

Optimizing the FI bottom sensitivity in the Infinite 200 PRO multimode reader series

Optimizing the luminescence sensitivity in the Infinite 200 PRO multimode reader series

Based on the successful Infinite 200 series, Tecan has developed the Infinite 200 PRO, with new enhancements, like the automated adjustable z-focusing in the Infinite M200 PRO. The adjustable z-focus for FI top measurements, which offers equally high sensitivity for all plate formats, helping to implement assay miniaturization. It is a flexible tool to customize measurement parameters to plate type and assay volume Z-focusing can be performed either by using the automated zcalculation function in the fluorescence intensity measurement stripe or by selecting the z-position option of the instrument menu in i-controlTM software. The z-position dialog of the instrument menu of Infinite M200 PRO provides an additional function which allows performing z-position scans for both a signal and a blank well for each fluorescent top measurement label. Beside the optimal z-position of signal and blank, the zposition for the maximum signal to blank ratio is also displayed. This automated z-adjustment with integrated background value correction is particularly suited to cell-based applications using autofluorescent growth media, providing automatic optimization of the signal-to-background ratio. The desired z-positions can be selected and applied to the measurement script by the user. In this technical note the impact of z-focusing on the sensitivity of fluorescence top measurements on Infinite M200 PRO is presented.

This Technical Note describes the successful
implementation of Invitrogen’s LanthaScreen TR-FRET assay system on Tecan’s Infinite F200 filter-based multi modular detection system. The Infinite F200 has performed according to Invitrogen’s LanthaScreen certification program criteria and was successfully validated by Invitrogen as “LanthaScreen® Certified”.

Scientists from Affymetrix® Inc., a world-leading supplier of microarray equipment and assays, evaluated the Tecan Infinite® 200 NanoQuant multimode microplate reader and two other spectrophotometers for DNA concentration analysis.
In this note, data are presented from a comparison done by Affymetrix using Tecan’s Infinite 200 NanoQuant, Molecular Devices SpectraMax® Plus and the Nanodrop® ND1000 instrument.

For measurement and differentiation of small amounts of RNA and DNA, two detection systems using fluorescent nucleic acid binding dyes have been tested - the Quant-iT PicoGreen® reagent to measure DNA and the RiboGreen® reagent for detection of small amounts of RNA.For detection and quantification of small amounts of dsDNA and RNA within a wide range of concentrations, the fluorescence intensity measurement with the Tecan Infinite® 200 series provides sensitive and accurate measurement results.