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Infinite® F500 - Typical performance values

 Light sources
  High energy xenon flash lamp
 Wavelength selection
  Filter-based
 Wavelength range
Absorbance 230 – 1,000 nm
Fluorescence Ex: 230 – 900 nm; Em: 280 – 900 nm
Fluorescence polarization Ex: 300 – 750 nm; Em: 330 – 750 nm
 Detectors
 Absorbance UV Silicon photodiode
 Fluorescence Extended wavelength (UV and far red) ;
Low dark current PMT
 Luminescence Photon counting; Low dark current PMT
 Plate formats
  6 – 1,536-well plates, NanoQuant Plate
 Temperature control
  Ambient +4 to 42 °C
 Shaking
  Linear, orbital with variable amplitudes
 Fluorescence sensitivity values
 Fluorescence top 10 amol/well fluorescein
(Greiner® 384-well low volume black plate; 10 µl)
 Fluorescence bottom 0.4 fmol/well fluorescein
(Greiner 96-well SensoPlateTM; 200 µl)
 TRF 0.5 amol/well europium
(Greiner 384-well low volume white plate; 10 µl)
 FP < 2 mP standard deviation @ 1 nM fluorescein
 Luminescence sensitivity values
 Glow luminescence* 225 amol/well ATP
(Greiner 384-well low volume white plate; 25 µl)
 Flash luminescence** 12 amol/well ATP
(Greiner 384-well white plate; 55 µl)
 Absorbance
 Measurement range 0 – 4 OD
 OD accuracy 0 – 2 OD: ≤ ± (1 % + 10 mOD)
 OD reproducibility 0 – 2 OD: ≤ ± (0.5 % + 5 mOD)
 Fastest reading times (on-the-fly)
 Fluorescence (kinetic cycle) 1,536-well: 26 sec 384-well: 19 sec
 Fluorescence (plate in/out) 1,536-well: 40 sec 384-well: 35 sec
 Injectors
 Injection volume Variable; 1 µl increments
 Syringe size Selectable: 500 / 1,000 / 2,500 µl
 Pump speed 100 – 300 µl/sec
 Dead volume 100 µl (including pump back)
 Accessories
 Stacker Connect
 Barcode scanner left or right


* 144-041 ATP detection kit SL (Biothema)
** ENLITEN® ATP assay system (Promega)

 

 


Overview
Infinite F500

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Related Topics
Comparison of Absorbance Readers
Comparison of Multimode Readers
List of all application notes
Application Guide

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