Nucleofection

Tecan offers a range of automated cell transfection systems that take care of the time consuming and laborious processes of transfecting different types of cells. The workstations perform automated cell transfection using either lipofection or Nucleofection®, a method based on a combination of special buffers and electrical parameters. 

All liquid handling steps are automated, from the preparation of cells and transfection mixes to incubation of the transfection plates. The systems ensure precise and accurate pipetting throughout the process, resulting in repeatable and consistent results.

The modular and scalable systems are based on Tecan’s flexible Freedom EVO liquid handling platform  including:

• cooled or heated devices for DNA/RNA solutions and for cell media
• clean bench or class 2 type cabinet
• fixed tip for large scale reagent dispensing
• disposable tip cone for 10 μl or 200 μl tips
• non-centric pipetting to avoid disrupting cell monolayers
• 96 or 384 multichannel pipettors
• integrated CO2 incubator for automating cell delivery and incubation
• integrated thermocyclers for PCR applications

The integration of the Nucleofector® 96-well Shuttle® System from amaxa
biosystems with the Tecan workstations allows the automation of high
throughput transfection in primary cells (e. g. T-Cells) and difficult-to-transfect
cell lines (e. g. Jurkat cells) for the first time.

Links to additional information:

High-throughput transfection and engineering of primary cells and cultured cell lines – an invaluable tool for research as well as drug development

Using Tecan’s Freedom EV0@ to produce a human genome-wide RNAi library (page 3)

RNAi Libraries for Functional Genomics

Movie explaining Nucleofection (not including automation)

Are you looking for your own customized solution? Do you want to integrate a specific device? Tecan Integration Group supports you in planning, realization and final installation of a tailor-made solution specific designed for your needs.

Scientific instrumentation. Not for use in human clinical or in-vitro diagnostic procedures.