Freedom EVO® Drug Metabolism

The Drug Metabolism platform can routinely perform a variety of in vitro assays, including metabolic stability, cytochrome P450 inhibitions, isoform identification, metabolite identification and cytochrome P450 induction assays. For microsome or hepatocyte metabolic stability assays, a choice of temperature-controlled shakers that keep microsomes or cells in suspension, and temperature-controlled racks or reagent carriers to handle pre-incubation or post-incubation processing steps can be included. Example of data generated on a Freedom EVO is shown in the table below.

Drug metabolizing profiling data

12-point dose response curve. Final dose range 100 μM – 2nM, DMSO control. IC50 (μM) listed (unless noted otherwise). + = positive cooperativity(stimulation).
NI = No inhibition. >100 = inhibition noted at higher concentrations.
Data courtesy of Promega, as presented by Tracy Worzella at 2006 SBS, Seattle. Data generated using Promega P450-610™ and MAO-610™ Assays. A bibliography for published values can be found in:
Cali JJ, Ma D, Sobol M etal. Luminogenic cytochrome P450 assays, Exp. Op. Drug Metab. Toxicol. (2006) 2 (4): 629-645.