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The HS Pro Hybridization Station offers several chamber formats for various microarray dimensions:

• Chambers with 1 active area
• Chambers with 2 active areas
• Chamber with 4 active areas

Chambers with single active area:

Chamber 51x20

• Area inside chamber sealing: 51 x 20 mm
• Suggested array dimensions (considers all system and slide tolerances)

			Dimension [mm]
			Min	Max
	Slide	A	25	26
		B	75.3	76.5
		C	0.8	1.2
Recommended area for spotting	Array	a	3.2
		b		18.8
		c	21.8
		d		45.9
	Barcode	e		15.6

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Chamber 57x21

• Area inside chamber sealing: 57 x 21 mm
• Suggested array dimensions (considers all system and slide tolerances)

			Dimension [mm]
			Min	Max
	Slide	A	25	26
		B	75.3	76.5
		C	0.8	1.2
Recommended area for spotting	Array	a	2.7
		b		19.8
		c	15.8
		d		51.9
	Barcode	e		9.6

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Chamber 63.5x21

• Area inside chamber sealing: 63.5 x 21 mm
• Suggested array dimensions (considers all system and slide tolerances)

			Dimension [mm]
			Min	Max
	Slide	A	25	26
		B	75.3	76.5
		C	0.8	1.2
Recommended area for spotting	Array	a	2.7
		b		19.8
		c	9.3
		d		58.4
	Barcode	e		3.0

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Chamber A1x244k (61.5x21)

• A1x244k chambers are designed for use with Agilent 244k arrays
• Area inside chamber sealing: 61.5 x 21 mm
• Suggested array dimensions (considers all system and slide tolerances)

			Dimension [mm]
			Min	Max
	Slide	A	25	26
		B	75.3	76.5
		C	0.8	1.2
Recommended area for spotting	Array	a	2.7
		b		19.8
		c	13
		d		57.9
	Barcode	e		8.5

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Chambers with dual active area:

Segmented Chamber: Dual Active Area Chamber 27.8x20

• Area inside chamber sealing: 27.8 x 20 mm
• Suggested array dimensions (considers all system and slide tolerances

			Dimension [mm]
			Min	Max
	Slide	A	25	26
		B	75.3	76.5
		C	0.8	1.2
Recommended area for spotting	Array	a	3.2
		b		18.8
		c1	45.6
		c2	13.7
		d		22.7
	Barcode	e		7.6
	Barcode	e		8.5

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A2x105k Chamber: Dual Active Area Chamber 28.2x21

• A2x105k chambers are designed for use with Agilent 2x105k arrays
• Area inside chamber sealing: 28.2 x 21 mm
• Suggested array dimensions (considers all system and slide tolerances)

			Dimension [mm]
			Min	Max
	Slide	A	25	26
		B	75.3	76.5
		C	0.8	1.2
Recommended area for spotting	Array	a	2.7
		b		19.8
		c1	45.4
		c2	13.7
		d		24.6
	Barcode	e		8.5
	Barcode	e		8.5

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QuadChamber

QuadChamber A4x44k (22x13)

• A4x44k Quad Chambers are designed for use with Agilent 44k arrays
• Area inside chamber sealing: 22x13 mm
• Suggested array dimensions (considers all system and slide tolerances)

			Dimension [mm]
			Min	Max
	Slide	A	25	26
		B	75.3	76.5
		C	0.9	1.1
Recommended area for spotting	Array	a	2.6
		b		19.8
		c1	12.6
		c2	28.3
		c3	44
		c4	59.8
		d		11.8
	Barcode	e		10.3

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