Freedom EVO® Protein Digest

Convenient and robust in-gel digestion and MALDI target spotting

The Tecan Freedom EVO protein digest workstation enables fully automated protein digest for subsequent MALDI-TOF analysis, entirely removing all manual steps for destaining of gel plugs, reduction, alkylation, in-gel trypsin digest, peptide extraction, and spotting onto MALDI targets. The system allows the processing of up to 192 samples per day, including MALDI spotting.

The entire process from destaining of gel plugs to peptide extraction is performed in the ABgene digest plate, which is handled through special frame adapters. This system was developed in collaboration with the Proteomics Group at the Max-Planck-Institute of Experimental Medicine in Göttingen, Germany (see Jahn et al., Anal. Bioanal. Chem. 2006, 386:92-103). It allows for:

Contact free addition and removal of agents

Minimal evaporation during incubation steps

Efficient drying processes

Cooling of the peptide mixture after digestion.

Characteristics include:

No piercing of gel-plug due to contact free liquid handling

Maximal protection against Keratin contamination

Optimized protocols leading to consistent results

Sensitivity down to 50 – 100 fmol of protein per gel plug

Sequence coverage equal or better compared to manual processes

Higher working efficiency due to low hands-on time

Cooling capability allows for overnight runs

Robust and reproducible results

Sequence coverage of BSA, automated (green) vs. manual (white) digest

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Freedom EVO Protein Digest

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The entire process from destaining of gel plugs to peptide extraction is performed in the ABgene digest plate, which is handled through special frame adapters. This system was developed in collaboration with the Proteomics Group at the Max-Planck-Institute of Experimental Medicine in Göttingen, Germany (see Jahn et al., Anal. Bioanal. Chem. 2006, 386:92-103). It allows for: Contact free addition and removal of agents Minimal evaporation during incubation steps Efficient drying processes Cooling of the peptide mixture after digestion.

Characteristics include: No piercing of gel-plug due to contact free liquid handling Maximal protection against Keratin contamination Optimized protocols leading to consistent results Sensitivity down to 50 – 100 fmol of protein per gel plug Sequence coverage equal or better compared to manual processes Higher working efficiency due to low hands-on time Cooling capability allows for overnight runs Robust and reproducible results		Sequence coverage of BSA, automated (green) vs. manual (white) digest