Freedom EVO® Expression Workstation

The generation of nucleic acid constructs and their expression in a suitable host relies on the reliable implementation of a range of processes.
Many of these processes are based on standardized kits supplied by the most renowned companies in the field. Tecan works together with these companies to jointly supply worry-free automation protocols.
Some of the applications that are run on the Freedom EVO robotic workstation:

• Restriction digestion
• Plasmid and other nucleic acid purification
• DNA/RNA quantitation and normalization
• E. coli transformation
• PCR/sequencing reaction setup
• Cell growth monitoring
• Ligation
• Cloning
• Determination of quality and quantity of protein expression
• Cell lysis
• Separation of soluble and insoluble protein fractions by centrifugation
• Purification and quantitation of proteins
• SDS-PAGE analysis (e-PAGE)

If you automate your expression protocols you can expect to:

• increase the chance of successful expression of functional proteins by highly parallel expression screening
• Reliably and repeatably process your protocols
• Minimize the hands-on time and release time for other tasks

An example of processes implemented on Freedom EVO to express proteins is presented in the following Article:Nature Methods; Published online: 3 May 2009 | doi:10.1038/nmeth.1326

A downloadable version of the protocols employed in this article can be found here.