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 | Extracted DNA performance in subsequent STR analysis |
0.1 µl of liquid blood was dried onto denim and stored at room temperature for 3 weeks. This spot was isolated using the DNA IQ System, and 5 µl of eluted material (of 25 µl total elution) was amplified in a standard PowerPlex® 16 amplification.
Sample lysates from positive controls or negative controls (blanks) were arranged in an alternating (checkeboard) pattern prior to extraction. 1 µl of a 1:3 dilution of the resulting DNA solution from positive controls or 10 µl of the undiluted blanks were processed through a PowerPlex 16® STR reaction, amplification and detection. Typical results are shown below for a DNA lysates and blank. No blanks exhibited peaks, indicating that the automated DNA IQ™ processing did not result in cross-contamination of samples.
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| 1 µl buccal swab, 1:3 dilution | 10 µl blank |
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