Freedom EVO® Process Development

Screening of chromatographic conditions

The new PreDictor™ 96-well filter plates prefilled with GE Healthcare BioProcess™ chromatography media have been fully tested for screening of chromatographic conditions on Freedom EVO platforms. The combination of Tecan's robotic integration and liquid handling expertise together with the versatile PreDictor platform of GE Healthcare gives the user a fully automated system for high throughput process development. The data obtained by parallel screening of chromatographic conditions using PreDictor plates show good correlation with data obtained using chromatography columns, making the system an excellent tool for initial screening of process conditions.

Accessories dedicated to PreDictor:

To ensure robust and reliable processing of PreDictor plates on Freedom EVO, dedicated accessories are available from Tecan. These include a Holder filter paper and the Plate shaker PreDictor frame, both important for steps that minimize the risk of leakage through the filter in the plate. The Holder filter paper is used to blot a PreDictor plate on a filter paper prior to incubation on Te-Shake™, thereby removing possible droplets from the bottom of the plate. The Plate shaker PreDictor frame fixes the PreDictor plate on a collection plate during incubation at 1,100 rpm, to prevent the filter of PreDictor plate from coming into contact with the surface underneath.

PreDictor plates shorten time-to-clinic and increase productivity by:

Reduction of experimental time from weeks to hours
Significantly lowered sample consumption
Increased process understanding due to the enlarged experimental space

Approved applications include:

Screening for chromatographic conditions, including:

Binding capacity (time-dependent / time independent)
Wash and elution conditions
Screening of different chromatography media

Links:

High-Throughput Process Development: Determination of Dynamic Binding Capacity Using Microtiter Filter Plates Filled with Chromatography Resin

Effect of salt concentration and pH on recovery and purity of a target protein, described in the document 'Bioprocess Yearbook' in the 'Downloads' section

Conceptual visualization of a workflow for process development. Parallel screening using PreDictor plates makes it possible to explore a huge experimental space for best conditions (left). Once these have been identified, fine-tuning and verification are carried out on columns using ÄKTAdesign™ systems (middle). Finally, scale-up is done under conditions in which a robust production scale process can be operated (right).

ÄKTAdesign, BioProcess and PreDictor are trademarks of GE Healthcare companies.

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Freedom EVO Process Development

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