Clone selection and -picking

Creating stable producing cell lines for research and production is still, to some extent, a chance process. It requires the generation of a range of suitable constructs, transfection of these into host cells and selection of appropriate clones. These clones must be of single cell origin, express the target protein in the right quality and quantity and grow rapidly in a simple medium. Achieving an efficient, stable producing cell line can take months, and is critical to the marketability of a protein-based therapeutics. Automation can help to decrease this development time, and helps to fulfill the regulatory requirements on data and process documentation.

Cell line development is the centerpiece of any biologicallyproduced therapeutic. Although the general workflow is well established, each individual case will require adaptations. Tecan, in collaboration with leading suppliers in specific fields, can provide solutions to automate most steps of the cell line development process, as well as automated solutions for cell culture processes, and software and hardware solutions for biobanking applications. Automated cell line development processes include:

Cloning: Automated DNA purification, PCR set up, PCR clean up, vector assembly, ligation and transformation of E. coli are standard processes and can be performed with chemistries from leading providers.

Transfection: Automated high throughput lipofection and Nucleofection® are well established.

Selection: The most critical and variable step of the process is selecting optimal clones from a pool of randomly generated cell lines. Automation can be applied to limited-dilution plating, plate replication, assessing single cell clone origin, clone picking, expression screening using ELISA, protein quantitation and a range of protein characterization methods.

Cell expansion: Can be performed on automation solutions such as Cellerity.

Are you looking for your own customized solution? Do you want to integrate a specific device? Tecan Integration Group supports you in planning, realization and final installation of a tailor-made solution specific designed for your needs.

Scientific instrumentation. Not for use in human clinical or in-vitro diagnostic procedures.