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| Reception and identification |
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Arriving samples are scanned after entering the system in racks and barcodes can be communicated to the LIS/LIMS to retrieve work orders or simply confirm arrival of the tube in the laboratory and create an inventory of samples. All common 1D barcodes can be read within the strip rack or 2D barcodes on a single tube when transporting it to the first module after entering the system.
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| Centrifugation |
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The integrated centrifuge guarantees consistent centrifugation quality for all samples that require centrifugation. Time, temperature, speed and acceleration and deceleration profiles can be set for each run individually provided a consistent requirement for the samples. Pre-centrifuged samples can be centrifuged again or bypass the centrifuge and move on to the following procedure.
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| Decapping |
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To maximize safety for your staff and increase process security, the onboard decapper takes caps and plugs off most commonly used primary tubes. The cap type is detected and the tube taken away from the worktable to avoid cross contaminating other samples or the environment in the system. An protective shield applied during the decapping step prevents any aerosols from entering the area where samples are handled on the worktable.
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| Tube inspection |
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The tube inspection unit with the patented “see through” feature reads the liquid level and determines the location of the separation between serum and separation gel or red blood cells. To maximize the reliability, it also features a rotating device to increase its ability to process all tubes presented to it. Based on the values provided by the tube inspection unit, the volume available can be calculated and subsequent pipetting can be performed most efficiently.
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| Fractionation & buffy coat extraction |
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After identification of the separation interface between the red blood cells and buffy coat/plasma by the tube inspection unit, the sample can be fractionated into plasma aliquots and/or buffy coat.
Tecan, in collaboration with the IBBL, has developed a method that uses spiral pipetting for highly efficient extraction of buffy coat. The Application Note Efficient buffy coat extraction explains how this method increases yields and reproducibility over the manual method.
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| Nucleic acid purification |
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Freedom EVO® - HSM workstation
Tecan and Promega are partnering to provide a uniquely efficient and reliable system for isolating genomic DNA from large volumes (1-10 ml) of blood, typically drawn into a standard 10 ml Vacutainer tube. The combination of the Freedom EVO, the ReliaPrep HSM and the ReliaPrep Large Volume HT gDNA Kit allows automated recovery of pure gDNA from up to 64 blood samples (each up to 10 ml) within eight hours.
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| Aliquoting |
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Pipeting into a secondary tube, which can be produced on board of the FE500pro, or into micro plates is performed by a multi channel arm with disposable tips or, if the process allows it, by fixed needles. The arm configuration can vary between 2/4/8 channels which can be individually configured to carry fixed or disposable tips. In addition to the capacitive liquid level detection for volume calculation, clot detection and liquid tracking during the aspiration, on the Freedom Evo based liquid handling arm, the MultiSense technology allows pressure monitored pipetting and a qualitative liquid arrival check.
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| Software |
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As each and every biobank follows different protocols, the software workflow is fully customizable. That includes sample handling, data generation, handling and storage as well as LIS/LIMS interfaces.
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