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Infinite® M1000 PRO - Typical performance values

 Light sources
  High energy xenon flash lamp, light-emitting diodes
 Wavelength selection
  Premium Quad4 Monochromators
  Absorbance Fluorescence
 Bandwidth Fixed, 5 nm Adjustable ≤ 300 nm: 2.5 – 10 nm;
> 300 nm: 5 – 20 nm
 Wavelength accuracy ± 0.3 nm ≤ 300 nm: ± 0.5 nm;
> 300 nm: ± 1 nm
 Wavelength reproducibility ± 0.3 nm ≤ 300 nm: ± 0.5 nm;
> 300 nm: ± 1 nm
 Wavelength range
  Absorbance Fluorescence FP
  230 – 1,000 nm Ex: 230 – 850 nm; Em: 280 – 850 nm Ex (LED):
470, 530, 590, 635 nm
Em (Mono):
280 – 850 nm
 Detectors
 Absorbance UV Silicon photodiode, 4-channel parallel reading
 Fluorescence (incl. FP) Extended wavelength (UV and far red) 
Low dark current PMT
 Luminescence Photon counting low dark current PMT
 Plate formats
  6 – 1536-well plates, NanoQuant Plate
 Temperature control
  Ambient +4 to 42 °C
 Shaking
  Linear, orbital, double orbital with variable amplitudes
 Fluorescence sensitivity values
 Fluorescence top reading 25 amol/well fluorescein
(Greiner® 384-well low volume black plate; 10 µl)
 Fluorescence bottom reading 0.6 fmol/well fluorescein
(Greiner 96-well SensoPlate™; 200 µl)
 TRF 1.5 amol/well europium
(Greiner 384-well low volume white plate; 10 µl)
 FP < 2 mP standard deviation @ 1 nM fluorescein
 Luminescence sensitivity values
 Glow luminescence* 225 amol/well ATP
(Greiner 384-well low volume white plate; 25 µl)
 Flash luminescence** 12 amol/well ATP
(Greiner 384-well white plate; 55 µl)
 Absorbance
 Measurement range 0 – 4 OD
 OD linearity 0 – 3 OD: R2 ≥ 0.999
 OD accuracy 0 – 3 OD: ≤ ± (1 % + 6 mOD)
 OD reproducibility 0 – 3 OD: ≤ ± (0.5 % + 5 mOD)
 AlphaScreen
 Detection limit ≤ 100 amol/well bio-LCK-P***
≤ 2,5 ng/ml Omnibeads****
 Uniformity ≤ 3% CV***
 Z' Value ≥ 0,9***
 Fastest read time < 2min (384-well plate)
 Fastest reading times – fixed wavelength (on-the-fly)
 Absorbance 1,536-well: 19 sec 384-well: 15 sec 96-well: 11 sec
 Fluorescence 1,536-well: 35 sec 384-well: 24 sec 96-well: 17 sec
 FP 1,536-well: 100 sec 384-well: 42 sec 96-well: 22 sec
 Fastest reading times – scanning (450 – 550 nm, 5 nm step)
 Absorbance 96-well: 35 sec
 Fluorescence 96-well: 115 sec
 Injectors
 Injection volume Variable; 1 µl increments
 Syringe size Selectable: 500, 1000, 2500 µl
 Pump speed 100 – 300 µl/sec
 Dead volume 100 µl including pump back
 Accessories
 Stacker built-in with up to 50 microplates
 Barcode scanner left or right


* 144-041 ATP detection kit SL (Biothema)
** ENLITEN® ATP assay system (Promega)
*** (P-Tyr-100 Assay Kit, PE # 6760620); (384-well small volume plates)
****(PE # 6760626); (384-well small volume plates)

 

 


Overview
Infinite M1000 PRO

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Related Topics
Comparison of Absorbance Readers
Comparison of Multimode Readers
List of all application notes
Application Guide

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