Infinite® M1000 PRO - Typical performance values
| Light sources | |
| High energy xenon flash lamp, light-emitting diodes | |
| Wavelength selection | ||
| Premium Quad4 Monochromators | ||
| Absorbance | Fluorescence | |
| Bandwidth | Fixed, 5 nm | Adjustable ≤ 300 nm: 2.5 – 10 nm; > 300 nm: 5 – 20 nm |
| Wavelength accuracy | ± 0.3 nm | ≤ 300 nm: ± 0.5 nm; > 300 nm: ± 1 nm |
| Wavelength reproducibility | ± 0.3 nm | ≤ 300 nm: ± 0.5 nm; > 300 nm: ± 1 nm |
| Wavelength range | |||
| Absorbance | Fluorescence | FP | |
| 230 – 1,000 nm | Ex: 230 – 850 nm; Em: 280 – 850 nm | Ex (LED): 470, 530, 590, 635 nm Em (Mono): 280 – 850 nm |
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| Detectors | |
| Absorbance | UV Silicon photodiode, 4-channel parallel reading |
| Fluorescence (incl. FP) | Extended wavelength (UV and far red) Low dark current PMT |
| Luminescence | Photon counting low dark current PMT |
| Plate formats | |
| 6 – 1536-well plates, NanoQuant Plate | |
| Temperature control | |
| Ambient +4 to 42 °C | |
| Shaking | |
| Linear, orbital, double orbital with variable amplitudes | |
| Fluorescence sensitivity values | |
| Fluorescence top reading | 25 amol/well fluorescein (Greiner® 384-well low volume black plate; 10 µl) |
| Fluorescence bottom reading | 0.6 fmol/well fluorescein (Greiner 96-well SensoPlate™; 200 µl) |
| TRF | 1.5 amol/well europium (Greiner 384-well low volume white plate; 10 µl) |
| FP | < 2 mP standard deviation @ 1 nM fluorescein |
| Luminescence sensitivity values | |
| Glow luminescence* | 225 amol/well ATP (Greiner 384-well low volume white plate; 25 µl) |
| Flash luminescence** | 12 amol/well ATP (Greiner 384-well white plate; 55 µl) |
| Absorbance | |
| Measurement range | 0 – 4 OD |
| OD linearity | 0 – 3 OD: R2 ≥ 0.999 |
| OD accuracy | 0 – 3 OD: ≤ ± (1 % + 6 mOD) |
| OD reproducibility | 0 – 3 OD: ≤ ± (0.5 % + 5 mOD) |
| AlphaScreen | |
| Detection limit | ≤ 100 amol/well bio-LCK-P*** ≤ 2,5 ng/ml Omnibeads**** |
| Uniformity | ≤ 3% CV*** |
| Z' Value | ≥ 0,9*** |
| Fastest read time | < 2min (384-well plate) |
| Fastest reading times – fixed wavelength (on-the-fly) | |||
| Absorbance | 1,536-well: 19 sec | 384-well: 15 sec | 96-well: 11 sec |
| Fluorescence | 1,536-well: 35 sec | 384-well: 24 sec | 96-well: 17 sec |
| FP | 1,536-well: 100 sec | 384-well: 42 sec | 96-well: 22 sec |
| Fastest reading times – scanning (450 – 550 nm, 5 nm step) | |
| Absorbance | 96-well: 35 sec |
| Fluorescence | 96-well: 115 sec |
| Injectors | |
| Injection volume | Variable; 1 µl increments |
| Syringe size | Selectable: 500, 1000, 2500 µl |
| Pump speed | 100 – 300 µl/sec |
| Dead volume | 100 µl including pump back |
| Accessories | |
| Stacker | built-in with up to 50 microplates |
| Barcode scanner | left or right |
* 144-041 ATP detection kit SL (Biothema)
** ENLITEN® ATP assay system (Promega)
*** (P-Tyr-100 Assay Kit, PE # 6760620); (384-well small volume plates)
****(PE # 6760626); (384-well small volume plates)
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