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Infinite M1000 PRO - Scientific citations

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Anal Chem. 2011 Feb 15,83(4):1307-14. Epub 2011 Jan 24.
Title Fluorescence near gold nanoparticles for DNA sensing..
Authors Cheng Y, Stakenborg T, Van Dorpe P, Lagae L, Wang M, Chen H, Borghs G.
Institution Imec, Kapeldreef 75, Leuven, Belgium.
Journal Anal Chem. 2011 Feb 15,83(4):1307-14. Epub 2011 Jan 24.
Abstract We investigated fluorescence quenching and enhancement near gold nanoparticles (GNP) of various sizes using fluorescently labeled hairpin DNA probes of different lengths.
Text A closed hairpin caused intimate contact between the fluorophore and the gold, resulting in an efficient energy transfer (quenching). Upon hybridization with complementary DNA, the DNA probes were stretched yielding a strong increase in fluorescence signal. By carefully quantifying the amount of bound fluorescent probes and the GNP concentrations, we were able to determine the quenching and enhancement efficiencies. We also studied the size and distance dependence theoretically, using both FDTD simulations and the Gersten-Nitzan model and obtained a good agreement between experiments and theory. On the basis of experimental and theoretical studies, we report over 96.8% quenching efficiency for all particle sizes tested and a maximal signal increase of 1.23 after DNA hybridization. The described results also demonstrate the potential of gold nanoparticles for label free DNA sensing
Pubmed ID 21261273
 
Antiviral Res. 2011 Jul,91(1):72-80. doi: 10.1016/j.antiviral.2011.04.014. Epub 2011 May 5
Title Development of Vaccinia reporter viruses for rapid, high content analysis of viral function at all stages of gene expression .
Authors Dower K, Rubins KH, Hensley LE, Connor JH.
Institution Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
Journal Antiviral Res. 2011 Jul,91(1):72-80. doi: 10.1016/j.antiviral.2011.04.014. Epub 2011 May 5
Abstract Vaccinia virus is the prototypical orthopoxvirus of Poxviridae, a family of viruses that includes the human pathogens Variola (smallpox) and Monkeypox.
Text Core viral functions are conserved among orthopoxviruses, and consequently Vaccinia is routinely used to study poxvirus biology and screen for novel antiviral compounds. Here we describe the development of a series of fluorescent protein-based reporter Vaccinia viruses that provide unprecedented resolution for tracking viral function. The reporter viruses are divided into two sets: (1) single reporter viruses that utilize temporally regulated early, intermediate, or late viral promoters, and (2) multi-reporter viruses that utilize multiple temporally regulated promoters. Promoter and reporter combinations were chosen that yielded high signal-to-background for stage-specific viral outputs. We provide examples for how these viruses can be used in the rapid and accurate monitoring of Vaccinia function and drug action.
Pubmed ID 21569797
 
Bioorg Med Chem Lett. 2011 Jun 30
Title Identification of a non-phosphorylated, cell permeable, small molecule ligand for the Stat3 SH2 domain .
Authors Page BD, Fletcher S, Yue P, Li Z, Zhang X, Sharmeen S, Datti A, Wrana JL, Trudel S, Schimmer AD, Turkson J, Gunning PT.
Institution Department of Chemistry, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON, Canada L5L 1C6.
Journal Bioorg Med Chem Lett. 2011 Jun 30
Abstract Signal transducer and activator of transcription 3 (Stat3) protein is a cytosolic transcription factor that is aberrantly activated in numerous human cancers. Inhibitors of activated Stat3-Stat3 protein complexes have been shown to hold therapeutic promise for the treatment of human cancers harboring activated Stat3.
Text Herein, we report the design and synthesis of a focused library of salicylic acid containing Stat3 SH2 domain binders. The most potent inhibitor, 17o, effectively disrupted Stat3-phosphopeptide complexes (K(i)=13µM), inhibited Stat3-Stat3 protein interactions (IC(50)=19µM) and silenced intracellular Stat3 phosphorylation and Stat3-target gene expression profiles. Inhibition of Stat3 function in both breast and multiple myeloma (MM) tumor cells correlated with induced cell death (EC(50)=10 and 16µM, respectively).
Pubmed ID 21788134
 
Biosci Rep. 2011 Mar 2,31(4):283-94.
Title Molecular determinants involved in activation of caspase 7.
Authors Boucher D, Blais V, Drag M, Denault JB.
Institution Department of Pharmacology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4
Journal Biosci Rep. 2011 Mar 2,31(4):283-94.
Abstract During apoptosis, initiator caspases (8, 9 and 10) activate downstream executioner caspases (3, 6 and 7) by cleaving the IDC (interdomain connector) at two sites. Here, we demonstrate that both activation sites, site 1 and site 2, of caspase 7 are suboptimal for activation by initiator caspases 8 and 9 in cellulo, and in vitro using recombinant proteins and activation kinetics.
Text Indeed, when both sites are replaced with the preferred motifs recognized by either caspase 8 or 9, we found an up to 36-fold improvement in activation. Moreover, cleavage at site 1 is preferred to site 2 because of its location within the IDC, since swapping sites does not lead to a more efficient activation. We also demonstrate the important role of Ile195 of site 1 involved in maintaining a network of contacts that preserves the proper conformation of the active enzyme. Finally, we show that the length of the IDC plays a crucial role in maintaining the necessity of proteolysis for activation. In fact, although we were unable to generate a caspase 7 that does not require proteolysis for activity, shortening the IDC of the initiator caspase 8 by four residues was sufficient to confer a requirement for proteolysis, a key feature of executioner caspases. Altogether, the results demonstrate the critical role of the primary structure of caspase 7's IDC for its activation and proteolytic activity.
Pubmed ID 20942802
 
BMC Biotechnol. 2011 Mar 25,11:27.
Title Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1?C expression strain..
Authors Noens EE, Williams C, Anandhakrishnan M, Poulsen C, Ehebauer MT, Wilmanns M.
Institution European Molecular Biology Laboratory (EMBL), Hamburg Outstation, c/o DESY, Building 25a, Notkestrasse 85, 22603 Hamburg, Germany
Journal BMC Biotechnol. 2011 Mar 25,11:27.
Abstract The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins.
Text Unfortunately, the Hsp60 chaperone GroEL1, which is relatively highly expressed, is often co-purified with polyhistidine-tagged recombinant proteins as a major contaminant when using this expression system. This is likely due to a histidine-rich C-terminus in GroEL1.
Pubmed ID 21439037
 
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