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The Tecan Freedom EVO liquid handling workstation enables fully automated parallel column chromatography for the first time, using Atoll’s MediaScout® RoboColumn system, entirely removing all manual steps from column equilibration to column regeneration. The system is a powerful new tool for process development, bioreactor monitoring and sample conditioning, by allowing valid chromatographic conditions to be used without sophisticated LC systems.

MediaScout® RoboColumns feature individual mini columns arranged on a 96-well format base plate, sealing to the fixed tips of the liquid handling arm through a pressure tight inlet (patents pending). The Freedom EVO platform controls flow rate and transfer volumes with accurate and precise positive liquid displacement to achieve highly reproducible chromatographic separations. Characteristics include: Choice of any commercially available resin Pre-packed in the volumes of 50, 100, 200 or 600 µl Bed heights of 2.5, 5, 10 or 30 mm, inner diameter 5 mm Flow rates adjustable between 16.2 cm/h and 1500 cm/h Simulation of gradients by using incremental buffer conditions Data points of fractions down to 25 µl combine to chromatograms Parallel processing of 8 columns simultaneously  Applications include: Resin and condition screening to determine optimum purification conditions Method development for transfer to larger scale purification Sample conditioning/purification/concentration for downstream applications like protein crystallography, ELISA or MS analysis. Bioreactor monitoring to determine the optimal harvesting point Links High Throughput Screening for the Design and Optimization of Chromatographic Processes - Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies Automated, High-Throughput Chromatographic Separation of Biological Compounds A microplate compatible HTS platform for parallelized small scale biochromatography

Superimposed chromatograms for the separation of 2 proteins from 8 RoboColumns (refer to the brochure for details)