Automation effectiveness in HLA typing
Automating the NGSgo library preparation workflow on the Freedom EVO NGS workstation generates high yield libraries. After sequencing, the number of reads assigned to each library was within 17 percent of the average. GenDx NGSengine® software generated HLA typing results for all samples and loci, demonstrating the effectiveness of this automated workflow for library preparation.
Sequencing metrics confirmed the generation of high quality libraries:
- sample mappability was over 85 percent for all samples
- locus mappability exceeded 90 percent
- all loci were fully covered with a read depth of at least 194 in the HLA core sequencing regions
- heterozygous positions were well balanced and cleanly separated from the sequencing noise