Lab automation solutions for coronavirus research and detection

Need to scale up your research?

Ready-to-go workflows with DreamPrep solutions for nucleic acid extraction and NGS library prep combining our Fluent Automation Workstation and Fluent Gx Assurance Software solution for a regulated environment.

Get started immediately with pre-programmed workflows for nucleic acid extraction using Zymo kits

The DreamPrep NAP workstation featuring Zymo Research simplifies your nucleic acid extraction workflows – today and in the future. It’s optimized workflow for the Quick- DNA/RNA™ Viral MagBead kit was developed and verified in close collaboration with Zymo Research, and allows purification of up to 96 samples in less than two hours.

DreamPrep NAP COVID-19

Zymo Research recently evaluated the Quick-DNA/RNA Viral MagBead kit in a workflow to detect SARS-CoV-2 – the causative agent of COVID-19. Figure 1 shows qPCR results (data obtained by Zymo Research following the kit instructions in a manual workflow).

A significant inventory of the Quick-DNA/RNA Viral MagBead kit is available to address increasing global demand for optimal viral RNA extraction tools.

Figure 1: RT-qPCR amplification plots for a clinical sample and controls using a SARS-CoV-2 assay.
A sputum sample (200 µl stabilized in 2x 200 µl of DNA/RNA Shield™ transport and storage medium) was purified using the Quick-DNA/RNA Viral MagBead kit and eluted in 50 µl of DNase/RNase-free water. From this purified sample, 2.5 µl were analyzed using RT-qPCR to test for the presence of SARS-CoV-2 using human RNase P, 2019 nCoV_N1 (CV-1), 2019 nCoV_N2 (CV-2), and 2019 nCoV_N3 (CV-3) primer sets. Purified HeLa RNA and purified HeLa RNA spiked with a known target were used as negative and positive controls, respectively. RNase P crossed the Ct value before 35 cycles, indicating that the sample is of acceptable quality for the test.

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Boost your productivity with DreamPrep NAP.

DreamPrep-NAP-Fluent-Bubbles

Is your extraction procedure delivering inhibitor-free viral DNA/RNA for highly sensitive downstream detection?

 

Learn how DreamPrep NAP can process your increased workload using Zymo’s Quick-DNA/RNA Viral extraction chemistry.

Click here to download this application note

NGS library preparation for coronavirus research

Tecan’s Trio RNA-Seq™ library preparation solution is a revolutionary approach to pathogen identification from low-input samples, delivering augmented information on viral abundance, mutation, spectrum surveillance and gene expression. This knowledge helps to determine disease incubation times, quarantine periods, the risks and impact of healthy carriers on the general population, epidemiology and viral evolution.

Trio RNA-Seq incorporates proprietary technologies that generate RNA-Seq libraries from precious, low-input samples, while depleting abundant uninformative sequences. These powerful technologies include:

  • Single Primer Isothermal Amplification (SPIA) enables access to limited and degraded samples as low as 500 pg. SPIA increases detection sensitivity, effectively addressing the challenge of low input and low viral titer, particularly in asymptomatic patients or healthy carriers
  • Enzymatic fragmentation and DimerFree library construction allows efficient and robust library preparation
  • AnyDeplete removes abundant uninformative sequences, such as human ribosomal RNA (rRNA), to maximize detection sensitivity without excessive sequencing

References:
1) Chen, L., Liu, W., Zhang, Q., Xu, K., Ye, G., Wu, W., … Liu, Y. RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak. Emerging Microbes & Infections, 2020, 9(1), 313–319. doi: 10.1080/22221751.2020.1725399
2) Shen Z, Xiao Y, Kang L, et al. Genomic diversity of SARS-CoV-2 in Coronavirus Disease 2019 patients. Clinical Infectious Diseases. September 2020. doi:10.1093/cid/ciaa203

References

Two recent peer-reviewed studies (published in 2020) demonstrate the successful application of the Trio RNA-Seq Library Preparation Kit in detecting the novel coronavirus in patient samples:

  • Researchers from Wuhan University, China, used the Trio RNA-Seq Library Preparation Kit to sequence virus genomes from limited COVID-19 patient samples. The genomes of SARS-CoV-2 were successfully analyzed to determine its origin, evolutionary history and virulence.
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  • Researchers performed metatranscriptome sequencing on bronchoalveolar lavage fluid from eight SARS-CoV-2 patients, 25 community- acquired pneumonia (CAP) patients and 20 healthy controls, to study viral evolution and determine how the virus interacts with other microorganisms in the lung. The authors concluded that the virus evolves after infection, which may impact its virulence, infectivity and transmissibility.
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Check out the poster on the performance of Trio RNA-Seq kit for viral detection in clinical samples.

Click here to download this poster

Automate your NGS library preparation now!

Looking for short response times in SARS-CoV-2 or other research areas?

DreamPrep NGS Fluent

DreamPrep NGS gets the job done. Obtain high-quality, sequencing-ready NGS libraries in hours, quantified at no sample loss and with either minimal manual interaction for RNA or fully walk-away for DNA.

DreamPrep NGS is a flexible and future-proof library preparation platform. The optimized worktable enables almost any NGS workflow while ensuring high speed and accuracy in automated library prep and quantification.

For research use only – not for use in diagnostic procedures.

The automated workflow for the Trio RNA-Seq Library Preparation Kit on DreamPrep NGS is coming soon.

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Need to ramp up sample processing?

High throughput? Fast turnaround? Speed up your clinical diagnostic workflows with the fastest liquid handler from Tecan—the Fluent Gx, a Class 1 medical device in the US.

Blazing fast sample prep for regulated environments

Your protocols are already defined and you “just need to run them?” Fluent Gx provides the workflow tools you need to run your LDTs or clinical workflows at scale.

Tackle COVID-19 research and screening head on with Fluent Gx designed for easy method adoption and fast protocol execution in regulated clinical environments.

Need to automate your liquid handling tasks?

Make the most of your resources with our connectivity and monitoring solutions

Introspect™

Understand your instrument usage and availability with the easy and secure cloud-based reporting and analysis service. Even when working remotely you get to view Instrument productivity and consumables consumption on a web-browser anywhere!

Tecan Connect™

Reduce the density of staff in the lab by monitoring your instrument on your smartphone with the Tecan Connect mobile app. Connect is a cloud-based laboratory automation monitoring app that is designed to work with all Fluent and Freedom EVO® instruments.

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