Zymo Research recently evaluated the Quick-DNA/RNA Viral MagBead kit in a workflow to detect SARS-CoV-2 – the causative agent of COVID-19. Figure 1 shows qPCR results (data obtained by Zymo Research following the kit instructions in a manual workflow).
A significant inventory of the Quick-DNA/RNA Viral MagBead kit is available to address increasing global demand for optimal viral RNA extraction tools.
Figure 1: RT-qPCR amplification plots for a clinical sample and controls using a SARS-CoV-2 assay.
A sputum sample (200 µl stabilized in 2x 200 µl of DNA/RNA Shield™ transport and storage medium) was purified using the Quick-DNA/RNA Viral MagBead kit and eluted in 50 µl of DNase/RNase-free water. From this purified sample, 2.5 µl were analyzed using RT-qPCR to test for the presence of SARS-CoV-2 using human RNase P, 2019 nCoV_N1 (CV-1), 2019 nCoV_N2 (CV-2), and 2019 nCoV_N3 (CV-3) primer sets. Purified HeLa RNA and purified HeLa RNA spiked with a known target were used as negative and positive controls, respectively. RNase P crossed the Ct value before 35 cycles, indicating that the sample is of acceptable quality for the test.