Sake studied biology at Utrecht University, and later specialized in the use and development of molecular tools in genetics. He has performed research in labs in the Netherlands, UK and USA, and published his work in high impact journals such as Cell and Blood. Sake obtained his PhD from UMC Nijmegen, where he studied the function of specific fusion genes in leukemia. He was also a post doctoral fellow in the Bernards lab at the Netherlands Cancer Institute. Sake joined the R&D department of GenDx at the start of 2017, where he develops diagnostic sequencing tools for HLA typing. Together with Tecan, he has automated the NGSgo library preparation workflow on the Tecan Freedom EVO.