How should I prepare the cell suspension for single-cell dispensing?

Cell suspension preparation protocol

1. Start by washing the cell in the flask with PBS two times, then add trypsin-EDTA. Rock back and forth to ensure trypsin-EDTA covers completely the cell monolayer.
2. Proceed by incubating the cell flask with trypsin-EDTA for 10 minutes at 37 °C
3. Remove flask from incubator and add 3 ml of complete media to neutralize trypsinization reaction
4. Using a serological pipette, pipette up and down 10 times to mix the cell suspension
5. Transfer 1 ml of cells to a sterile microcentrifuge tube
6. Determine cell viability and concentration using a cell counting method (automated cell counter, hemocytometry, etc.)
7. Normalize the concentration of your cells to 200 cell/µl
8. Dispense the desired number of cells in each well with Uno

 Further information

You can find further information about what can be dispensed with the Uno in the ‘Dispensing software user guide’, which can be accessed from the UnoControl software through File > Documentation > Dispensing Software User Guide > Appendix E.

Further help  

For further help, please refer to our Helpdesk.  

Link to Tecan Product Page:  

Uno Single Cell Dispenser™  

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