Manual serial dilutions are time and compound intensive, and often limit miniaturization efforts to a 96-well format. The D300e Digital Dispenser allows you to overcome the limitations of manual dilutions by dispensing compounds directly from the stock to the plate. This digital dispenser allows you to miniaturize your assay to 394 or 1,536-well plates, and prepare plates in minutes instead of hours, avoiding the toxic effects of DMSO on cells.
The smaller the minimum dispense volume, the larger the dynamic range of the dose response curve, and the lower the local and final DMSO concentrations. Below is a comparison of the effect on cell viability when 2 µl of 100 % DMSO are added to a cell layer with manual pipetting vs D300e Digital Dispenser (viable cells: green, dead cells: red).
This innovative dispense technology delivers compounds directly from the compound stocks, allowing you to fill an entire well plate in just minutes. Compare the times and compound consumption in our examples below to your current processes, and discover the advantages of the D300e Digital Dispenser.
Plate: 96-well, 100 µl/well assay volume
Samples: 8, 8 (10 mM stock concentration)
Concentrations: 12 12 (1.3-100,000 nM)
Time: 2.5 minutes
Compound: 2.1 µl/compound
Plate: 384-well, 60 µl/well assay volume
Samples: 11, (10 mM stock concentration)
Concentrations: 12 (2.7-100,000 nM)
Time: 3.5 minutes
Compound: 3.6 µl/compound
For research use only. Not for use in diagnostic procedures.
Tecan D300e Digital Dispenser is a product of HP Inc. CA, USA.
Contact us today and secure 1 hour remote software demo and 1 week free instrument trial in the lab to experience the benefit the D300e will bring to your research.