The NGS Transcriptomics team at AstraZeneca partners with scientists across AstraZeneca and externally, to enable transcriptomic analysis in various projects across our therapy areas. Björn will present how the team applies automation for its work, describing how they developed from low throughput, all manual work to utilizing automated liquid handling for their core processes. These include preparation of high quality RNA from various samples, sample normalization and randomization, library preparation and pooling of samples. Particular focus will be given to protocols for automated NGS library preparation, which have been established on Fluent 1080 system. These are full-day protocols that can process 8-96 samples through multiple enzymatic reactions, on-deck PCR runs, bead-based purifications, etc. He will also discuss the impact of automation on the team’s work in terms of quality and capacity, exemplifying with some recent projects.
