Viral pathogens like SARS-CoV-2 can be detected by RT-PCR in nasal or throat swab samples from patients with COVID-19, but it is the detailed, sequence-based analysis that provides an understanding of the nature and mutation of the virus and the resulting disease state. Next generation sequencing (NGS) offers a comprehensive view of the complete genome, providing information about viral origins, pathogenesis, genetic variations, immune responses and phylogenetics.
Starting samples for pathogen sequencing are almost always of variable quality, with at least some samples degraded or low concentration, and some inevitably falling below the detection threshold of many current amplification technologies.
The detection sensitivity and coverage across a broad range of viral loads is shown here.
Results successfully demonstrate the high detection sensitivity of SARS-CoV-2 using Revelo RNA-Seq, from as low as ~500 viral copies.
For the competitor kit, viral coverage drops drastically, even when the viral copy number is as high as ~200,000.