High-throughput, reproducible
organoid analysis made easy.

Save time and elevate your data quality with a compact 3-in-1 workstation
built for effortless 3D live-cell analysis.

 

MAKE THE MOVE TO 3D

Monitor every cellular event as it happens with Spark® Cyto

Scaling 3D culture systems manually is time-intensive and difficult to reliably reproduce. Spark Cyto combines microplate reading, cell imaging, and incubation in a single instrument to make 3D cell culture automation simple.

Start your protocol and walk away while Spark Cyto tracks organoid morphology, growth, and other key indicators, so you can step in only when the experiment requires.

  • Keep 3D cultures viable for days with protection against evaporation and full control of temperature and CO2/O2 levels
  • Save time and improve data quality by tracking growth curves and other key experimental parameters label-free in real time, no staining required
  • Simplify live-cell kinetic studies and maximize hands-off time with integrated lid handling and automated reagent dispensing

Go from images to insights faster

Eliminate artifacts and increase reproducibility

Stitching artifacts interfere with analysis and interpretation of organoid behavior.

Capture whole 96- or 384-wells in a single image and visualize detailed 3D structures with automated Z-stacking and projection, artifact-free.

3D analysis made easy

Automatically segment 3D objects using AI-powered analysis with the press of a button, in brightfield and fluorescence.

Analyze growth, viability and key morphological parameters without lengthy programming steps or booking core lab time. Standardize analysis across users, even without expert knowledge.

Get more data from your samples, faster

Enhance data quality and save time by multiplexing experiments across modes.

Combine image analysis with well-based plate reading techniques such as luminescence for measuring ATP levels.

Automated organoid analysis, start to finish

Take our 3-minute tour to learn how you can use Spark Cyto’s effortless imaging and analysis tools to advance your organoid research.

Standardize your cell culture workflow with DreamCell™

Achieve reliable, image-driven decision making and enhance assay reproducibility with the DreamCell platform, an integrated workstation that combines imaging and analysis capabilities with precise, high-throughput liquid handling.

DreamCell enhances reproducibility at scale by automating repetitive and time-consuming cell maintenance tasks, eliminating user variability. This allows you to focus less on routine work and more on advancing your research and making impactful decisions.

  • Ensure long-term cell culture consistency by combining automated imaging, environmental control, and precise pipetting in a single platform.
  • Increase reproducibility and eliminate human bias by relying on precise image-based monitoring to determine when your cells are ready for the next step.
  • Save time with walkaway automation using Spark Cyto’s real-time analysis, which can directly trigger actions like medium exchange and cell harvesting, eliminating the need for manual monitoring and interventions.
  • Enable anyone to generate high-quality 3D models regardless of their level of organoid experience.
  • Grow with confidence on the Fluent’s flexible, modular deck layout, allowing you to expand your workcell as your needs evolve.

READY TO SCALE?

Discover how customers use Spark Cyto and DreamCell to advance their organoid research

Personalized Cancer Screening Research

Find out how Predictive Oncology used Spark Cyto to enable label-free monitoring of drug responses in breast, colon, and ovarian tumor spheroids derived from cryopreserved tumor cells.

Endometriosis Drug Discovery

Explore how GynQura used Spark Cyto to screen drug candidates for endometriosis, combining automated cell handling with 3D imaging and luminescence readouts.

Stem Cell Automation

See how The Lions Eye Institute combined Spark Cyto and Fluent to automate stem cell culture and screen nearly 300 drugs in iPSC-derived retinal organoids.

Hepatotoxicity in 3D

See how SUN Bioscience multiplexed 3D assays with Spark Cyto-based imaging to reveal CYP enzyme activity in liver spheroids, better reflecting human physiology.

Make the move to 3D today

 
Complex in vitro models are reshaping research. Contact our application scientists to explore how Spark Cyto and DreamCell can advance your organoid experiments.

Frequently Asked Questions

Is Spark Cyto compatible with my culture formats?

In addition to 2D culture formats, Spark Cyto supports a wide range of 3D formats from 6-well to 384-well plates, including ultra-low attachment plates and microcavity well plates. It’s compatible with spheroids, organoids, tumoroids, organ-on-a-chip systems and other 3D culture formats.

Do I need microscopy expertise to use Spark Cyto?

No. Spark Cyto is designed to capture high-quality images at 2x, 4x, and 10x magnification without specialist microscopy training. It automates image acquisition including Z-stacking and projection, as well as AI-based analysis for intuitive, user-friendly imaging and analysis.

Can Spark Cyto support my experimental needs?

Spark Cyto is well-suited for workflows that require high-throughput viability assessment, growth rate monitoring, and kinetic studies across large compound libraries. Its whole-well imaging capability and multimode detection (including UV-Vis spectroscopy, fluorescence, and luminescence) make it flexible across experiment types.

Is Spark Cyto suitable for pharma and CRO environments?

Yes. Spark Cyto can support the full drug discovery continuum, from hit identification and validation through lead optimization. Its high-throughput imaging, multimode detection, and programmable protocols make it suitable for screening libraries against 3D organoid and tumor models within a scalable, reproducible automated pipeline.

How can I automate my workflow with Spark Cyto?

Spark Cyto integrates with a wide range of automation solutions, enabling semi-automated to completely automated workflows. It is designed to connect with reagent and cell dispensers and incubators or you can start with Fluent DreamCell, our end-to-end automated platform, all managed code-free.

How does Spark Cyto increase reproducibility?

Spark Cyto combines cell imaging with multimode detection to monitor the same cell sample over time while assessing multiple cellular parameters simultaneously. Integrated automation features, including lid handling and reagent dispensing, together with unique capabilities such as whole-well high-dynamic-range imaging, reduce hands-on time and maximize experimental reproducibility. Integration with the DreamCell platform enables full 3D cell culture automation and standardization.

What environmental controls does Spark Cyto offer?

Spark Cyto protects samples against evaporation and provides full control over temperature (from RT to 42 °C) and CO2/O2 levels, ensuring cultures remain healthy for days without manual intervention.

Have a question we haven't answered, or need a custom solution?

Every lab is different. If you have a workflow that doesn't fit a standard configuration, our team can help you design a solution that does. Get in touch to speak with a specialist.

*Spark Cyto and DreamCell Workstation are for research use only. Not for use in diagnostic procedures.