The prep determines the result.

Automate your proteomics sample prep workflows with Fluent®.

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Support for your entire workflow

Fluent brings together liquid handling, integrated devices and workflow control, so you can automate the essential steps in proteomics sample preparation.

Speed up sample prep by 3.5 - 4.5 hours per assay, reducing bench burden by up to two days per week by automating:*

Lysis or denaturation across a wide range of biological species
In-solution processing from reduction, alkylation to tryptic digestion
Magnetic bead-based protein capture and digestion
Automated peptide desalting and clean-up by processing SPE cartridges

Dry-down peptide samples for subsequent resuspension
Absorbance & fluorescence based protein/peptide quantification and normalization
Randomization and sample tracking across the workflow

*Source: Liu et al., “Automation streamlines peptide map preparation, analysis and reporting for biotherapeutic antibody characterization,” Talanta 298, 128959, 2026. doi:10.1016/j.talanta.2025.128959.

Consistency starts in sample prep

Error-prone sample prep can cause you to miss your next discovery.

Ensure the quality of your data by automating your sample prep, from biological species to ready-to-inject peptide samples.

Sample prep on Fluent protects sample integrity, reduces variability and delivers consistently high-quality results.

Figure A - consistent protein identification from plasma across days
Figure B - low variation in protein quantitation, with less than 6% CV attributed to automated handling
Figure C - biological integrity and data comparability between manual and automated workflows
Figure D - no cross-contamination between adjacent wells
Figure E - reliable preparation of low-concentration peptide samples, comparable to conventional preparation

Figures
A, B, D: AppNote: SIMPLIFIED, AUTOMATED SAMPLE PREPARATION FOR PLASMA PROTEOMICS, 403005 V1.0
C: HIGH THROUGHPUT SAMPLE PREP FOR EXTENSIVE PLASMA PROTEOMICS, 402943
E: AUTOMATED EVOTIP PURE LOADING USING THE FLUENT® AUTOMATION WORKSTATION, 403393

Preconfigured for discovery

The Proteomics Fluent gives labs a ready-to-run automation starting point for proteomics sample preparation, combining the modules, methods, and workflow control needed to move from setup to standardized execution faster.

Increase throughput and process up to 96 samples with the Flexible Channel Arm™, Multiple Channel Arm™ 96, and Robotic Gripper Arm™
Use on-deck thermal modules for in-solution digestion and a magnet for high-throughput bead-based protein capture in 96-well format
Automate cartridge-based peptide desalting with the positive pressure device Resolvex® i300

Normalize samples automatically using an integrated reader for peptide quantification
Start right away with pre-scripted methods to facilitate commonly used proteomics workflows
Minimize sample loss from adhesion or dead volumes using labware-optimized protocols
Manage your workstation code-free using the graphical user interface of Fluent Control™ with GxP support, TouchTools™ and Introspect™ for monitoring and error detection

Compatible with your sample prep needs

Start with pre-programmed methods for commercially available kits, then adapt them to fit your proteomics workflow.

Vendor	Kit	Qualification Method	Starting Material	Output
PreOmics®	ENRICH-iST	Mass Spectrometry	Human Plasma	Desalted peptides in ELUTE buffer
Evosep	Loading of Evotip Pure™	Mass Spectrometry	HeLa Digest	Peptides loaded onto Evosep Evotip Pure™
Pierce™	Quantitative Fluorescence Peptide Assay #23290	Fluorescence Readout	HeLa Digest	Normalized peptides in Solvent A

*Each pre-programmed protocol is open, editable and can be adapted to fit your workflow.

Find out what’s possible with Proteomics on Fluent

Extend automation across discovery workflows

Build on your proteomics workflows with tools that support upstream discovery, sample preparation, quantification and workflow control.

Frequently Asked Questions

What is the difference between a Fluent and the Proteomics Fluent?

The Fluent Automated Workstation by Tecan is an advanced laboratory automation platform designed to streamline liquid handling and sample preparation workflows. It combines robotic precision, flexible deck configurations, and intelligent software to improve efficiency and reproducibility in research and diagnostic laboratories. The Proteomics Fluent is based on the Fluent, and includes a defined configuration combining AirFCA, MCA96, RGA and integrated laboratory devices, with pre-scripted application methods for proteomic sample preparation.

What software is included and do I need to know how to code?

No coding experience is required. The Fluent platform runs on FluentControl™, an intuitive software environment that lets you build, configure, and optimize automated assays through a visual interface. Introspect™, Tecan's cloud-based monitoring tool, provides real-time visibility into instrument productivity and helps identify bottlenecks across your lab. For pre-packaged solutions, a library of scripts for common proteomics workflows is provided and continuously updated.

Does Fluent support GxP compliance?

Yes. The Fluent platform includes built-in support for regulated environments, including a full GxP audit trail, electronic signatures, and user access controls through FluentControl Gx Assurance software. The system maintains a single chain of custody from sample receipt through to analysis and Introspect™ provides automated error detection to support data integrity.

What training and support resources are available?

Tecan Academy offers both virtual and on-site training programs, delivered by a network of 70 certified instructors. With 45+ years of experience in laboratory automation and a service network spanning 52 countries, Tecan provides support across the full lifecycle of your system, from installation and method development to ongoing troubleshooting. Our team has expertise across the entire LC-MS workflow, from sample prep and protein purification to regulatory strategy.

Have a question we haven't answered or need a custom solution?

Every lab is different. If you have a workflow that doesn't fit a standard configuration, our team can help you design a solution that does. Get in touch to speak with a specialist.