Worklisting is a way for users to upload a .csv or .gwl file that has the desired pipetting, and FluentControl™ will automatically create a subroutine based on those parameters.
What is a Worklist?
A Worklist is a file containing pipetting instructions. FluentControl allows users to upload Worklists in. csv or. gwl formats to automatically create a subroutine based on those parameters through the Worklist function. The Worklist commands consist of individual lines (or records) and there are seven different record types available: Aspirate, Dispense, Wash Tips / Replace DiTis, Flush, Break, Set DiTi Type, and Comment. Each record starts with a single character indicating the type and is followed by one or more parameters, separated by semicolons. Some parameters are optional, and some record types have no parameters
Main Commands in Worklisting
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Convert CSV to GWL: This command allows users to convert a. csv (Comma Separated Value) file to a. gwl (Gemini WorkList) file. This is useful if the pipetting information is from an Excel file or not already in a. gwl format. Users can choose a file to import and specify the destination for the generated. gwl file. They must also specify which columns of the. csv correspond to which type of information, such as volume or source labware.
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Load Worklist: Once a Worklist with a. gwl file is available, it can be loaded using this command. Users can specify the arm used, liquid class, tips (Dynamic DiTi handling is optional), and waste and wash settings.
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Execute Worklist: This is the final step. It processes the output of all Load Worklist commands executed since the start of the script or since the last Execute Worklist command, in the chronological order they were loaded
Additional Settings and Parameters
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If Labware Not Found on Worktable: This drop-down list specifies how to react if the Worklist contains labware barcodes or labels not located on carriers accessible to the pipetting arm. Options include:
- Skip entries without warning: Skips any affected transfers from the Worklist.
- Show warning: Skips the affected Worklist and shows details of the skipped transfers in the trace view as a warning. The run will finish with warnings, and if electronic signatures are enabled, the operator must confirm these warnings at the end of the run.
- Show error message: Interrupts execution of the Worklist and shows an error message to the operator. The operator can choose to ignore and continue (the run will finish with errors) or abort execution of the script.
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Worklist Path and File Name: Specify the file name and location of the Worklist file (*.gwl). Enter the path and file name directly or click Browse to choose an existing file. This parameter accepts the name of a GWL text file in the form of an expression (either a fixed value or script variables can be used).
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Ignore GWL File Content Until Run: Use this checkbox to indicate whether the GWL file contents should be examined as part of a context check of the Load Worklist command. This is useful when the GWL file is not dynamically created or modified during script execution. The default state for a new “Load Worklist” command is selected.
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Tip Selection: Select the tips you want to use. If the Worklist file specifies the tip selection (with the TipMask parameter), it overrides the settings made here. If the commands in the Worklist specify a dedicated tip (with the TipMask parameter), this tip must be part of the tip mask set in the command. Mixed tip configurations (fixed tips and DiTis) are not allowed in the same Worklist.
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Liquid Class: Select a liquid class from the list of currently known liquid classes to be used as default (if not explicitly specified in the GWL file) or use a variable. You can choose a different liquid class for each source labware and destination labware.
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GWL File Uses Well Index Numbers: Uncheck this box if the GWL file contains alphanumeric well numbers (A1, A2, …, H11, H12) instead of numeric well numbers (1, 2, …, 95, 96). The box is checked by default, expecting numeric well numbers.
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Skip Wash / Disposable Tip Replacement Before Worklist: By default, the execution of a Worklist begins with a Wash (for fixed tips) or a DiTi replacement (Drop Tips followed by Get Tips, for DiTis). You can skip this default behavior by selecting this checkbox. The checkbox is cleared by default.
Further Help
For further help, please refer to our Helpdesk.
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