Differences between the available blanking options in the Nucleic Acid Quantification and Labelling Efficiency apps
Individual blanking:
Individual blanking means that each well that is used for subsequent sample measurement must be measured with a blanking buffer beforehand. Individual blank values are thereby assigned to each used well, and samples are blanked with the appropriate, well-specific blank value.
Individual blanking is generally recommended because it produces more accurate and reliable results.
Existing blanking results are discarded whenever the instrument is disconnected.
Average blanking:
Average blanking requires at least two or more wells to be measured with a blanking buffer. An average blank value is calculated and used for the blank reduction of subsequently measured samples wells. Average blanking can be used as a quality control for the NanoQuant Plate™ to identify wells with strongly divergent background absorbance. Due to the subtraction of an average, average blanking is less accurate than individual blanking. The percent deviation of the wells used for average blanking must be <10% CV, and the blanking measurement must be repeated if the CV is >10% before the wells are usable for sample measurement.
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