Maximum stock concentrations that can be dispensed using Uno
The concentration limits are:
- 100-200 cells/µl (C1a cassettes)
- 10 mM for small molecules up to 1,000 Da
- 1-3 mg/ml (T1 cassettes) or 0.3 mg/ml (D1 cassettes) for proteins up to 300 kDa, or DNA up to 10,000 base pairs
For T1 cassettes, biomolecule concentrations up to 3 mg/ml can be dispensed. When using T1 cassettes for bulk dispensing, where no dispensed volume is below 1 nl, the loading volume can be raised to 20 µl for biomolecule concentrations up to 1 mg/ml.
Please note that, if you are dispensing your sample in a mixture with other biomolecules (eg. albumin), the total concentration of biomolecules must be considered (eg. [sample] + [albumin]). The concentration of your small molecules, proteins or nucleic acids influences the viscosity of the liquid. At concentrations above the indicated thresholds, the viscosity impacts the dispensing performance.
Further information
You can find further information about what can be dispensed with the Uno in the ‘Dispensing software user guide’, which can be accessed from the UnoControl software through File > Documentation > Dispensing Software User Guide > Appendix E.
Further help
For further help, please refer to our Helpdesk.
Link to Tecan Product Page:
402951-005