Which buffers can I use to dispense my compounds with the Uno Singe Cell Dispenser?

Buffers that can be used with the Uno

1. The Uno allows you to dispense compounds dissolved in different buffers, as listed below.

DMSO, from 70 to 100 %
Acetonitrile
Water-based (aqueous) reagents such as:
- PBS, Tris-HCl, etc…
- Iodoacetamide
- Trypsin in Acetic acid
- TFA
- Formic acid
- TCEP
- DTT
- Hydroxilamine
- TEAB

Water-based (aqueous) buffers with the following concentrations of surfactants in the stock solution:
- Brij®-35, 0.1 %
- Triton™ X-100, 0.1 %
- TWEEN® 20, 0.3 % (recommended for cell-based assays)

If working with water-based buffers, please note that the maximum concentration of the total buffer components (eg. Tris HCl, PBS, etc...) should not exceed 150 mM

2. Further information

You can find further information about what can be dispensed with the Uno in the ‘Dispensing software user guide’, which can be accessed from the UnoControl software through File > Documentation > Dispensing Software User Guide > Appendix E.

Further help 

For further help, please refer to our Helpdesk. 

Link to Tecan Product Page: 

Uno Single Cell Dispenser™ 

402951-002