Walk-Away Monitoring of Cytotoxicity, Viability and Apoptosis
SIMPLIFYING CELL ANALYSIS WORKFLOWS WITH THE SPARK® 10M multimode reader.
Assaying cells in an appropriate window of time after a compound-mediated cytotoxic event can be extremely challenging. Missing this window may mean the difference between defining an effect as apoptotic or necrotic and can affect interpretation of a compound’s mode of action.
Monitoring membrane integrity changes and cell viability can reveal an exact point in time when primary or secondary necrotic events occur. Further multiplexing with a caspase-3/7 assay can help differentiate membrane breakdown due to apoptosis and secondary necrosis from an overt cytotoxic event.
This application note describes a method for kinetic monitoring of cytotoxicity and viability, using three Promega assays for viability, cytotoxicity and apoptosis assessment and Tecan’s Spark® 10M multimode reader.
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