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|Research scientists at the Max Planck Institute for Molecular Genetics in Berlin have developed an automated, high throughput sample preparation protocol for peptide mass fingerprinting, using a Tecan Te-MO™ pipetting robot, integrated within a Tecan liquid handling workstation. The preparation of samples is a critical limiting factor in MALDI-TOF mass spectrometry, but Dr Niklas Gustavsson and colleagues have used the Te-MO to achieve a simple, robust and reproducible method of sample preparation, reliably and in a highly automated manner.|
The Te-MO pipetting head dispenses the samples from eight 96-well microplates to two MALDI sample plates, each with 384 sample positions, and handles all washing stages automatically. To achieve high sensitivity, impurities such as salts and buffer components must be washed away efficiently with minimal loss of analytes. The Te-MO washes the samples including an effective rinsing process, to reduce pipette tip consumption and minimize cross contamination. Comparisons between pairs of replicate MALDI sample plates have demonstrated the high reproducibility of robotic handling by the Te-MO.
Tecan (www.tecan.com) is a leading global player in the Life Sciences supply industry specializing in the development, production, and distribution of advanced automation solutions enabling pharma and academic research, diagnostics, food and veterinary research as well as forensics. Through its REMP subsidiary (www.remp.com), Tecan is the leading supplier of compound and sample storage, management and logistics solutions. Tecan clients are pharmaceutical and biotechnology companies, university research departments and diagnostic laboratories. Founded in Switzerland in 1980, the company has manufacturing, research and development sites in both North America and Europe and maintains a sales and service network in 52 countries. In 2005, Tecan achieved sales of CHF 344.9 million (USD 275.9 Mio.; EUR 222.5 Mio.). Registered shares of Tecan Group are traded on the SWX Swiss Exchange (TK: TECN / Reuters: TECZn.S / Valor: 1210019).
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