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July 14, 2015 | Customer News

Nanoliter liquid transfers with Tecan’s Freedom EVO®

July 6, 2015

Nanoliter liquid transfers with Tecan’s Freedom EVO®

The Screening Center’s Freedom EVO offers large-scale screening for a variety of projects

Männedorf, Switzerland, July 6, 2015 – Researchers at Germany's University of Konstanz have established an innovative technique to allow more cost-effective screening of small molecules. Using the Freedom EVO® workstation’s MultiChannel Arm™ 384, the University’s Screening Center has achieved reliable liquid transfers down to just 80 nanoliters. Silke Müller, a biologist in the screening facility, explained: “We work with a wide range of samples – enzymes, mammalian cells, bacteria – using various high throughput screening assay formats according to the needs of individual research groups. Our Freedom EVO therefore needs to be very flexible in the types of assays we can perform.”

With a library of over 38,000 compounds for screening, the center wanted to avoid the need for wasteful pre-dilution of stock solutions to enable large-scale studies to be performed more cost-effectively. Silke continued: “The minimum transfer volume specified for the MCA 384 is 500 nl, but we needed to achieve reliable transfer of just 80 nl of liquid to meet our target final concentration per well. Using fixed steel tips, I developed a technique similar in principle to a pin tool device, relying on the residual liquid volume on the inside and outside of the tips following several aspiration and dispense cycles to provide very low volume transfers. Although the exact aspiration/dispense volume and number of cycles needs to be optimized for each assay buffer, this method has proved highly reliable, with CVs of ~10 % for 80 nl transfers.”

To find out more about Tecan’s drug discovery solutions, visit www.tecan.com/drugdiscovery

 
For more information please contact

Tecan Trading AG
Antonietta Allocca
Seestrasse 103
CH-8708 Männedorf

Tel +41 (0)44 922 81 11
Fax +41 (0)44 922 81 12

info@tecan.com
www.tecan.com



About Tecan

Tecan (www.tecan.com) is a leading global provider of laboratory instruments and solutions in biopharmaceuticals, forensics and clinical diagnostics. The company specializes in the development, production and distribution of automated workflow solutions for laboratories in the life sciences sector. Its clients include pharmaceutical and biotechnology companies, university research departments, and forensic and diagnostic laboratories. As an original equipment manufacturer (OEM), Tecan is also a leader in developing and manufacturing OEM instruments and components that are then distributed by partner companies. Founded in Switzerland in 1980, the company has manufacturing, research and development sites in both Europe and North America and maintains a sales and service network in 52 countries. In 2014, Tecan generated sales of CHF 400 million (USD 437 million; EUR 331 million). Registered shares of Tecan Group are traded on the SIX Swiss Exchange (TECN; ISIN CH0012100191).