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June 9, 2015 | Customer News

Tecan’s Freedom EVO® ideal for automated high throughput cell separation

June 9, 2015

Tecan’s Freedom EVO® ideal for automated high throughput cell separation

Jürgen Hubbuch, Sarah Gretzinger and Sarah Zimmermann in front of the Freedom EVO 200

Männedorf, Switzerland, June 9, 2015 – The Karlsruhe Institute of Technology has automated high throughput cell separation protocols using aqueous two-phase systems (ATPS) on a Freedom EVO® 200 liquid handling platform. PhD student Sarah Zimmermann explained: “ATPS offers many advantages compared to antibody-based methods of cell separation; cost-effectiveness, scalability and very mild, gentle conditions for purification of cell products. Initially, we performed ATPS manually, but this is tricky and can give rise to large errors and unreproducible data. Automation was the way forward.”

“The Freedom EVO workstation has enabled us to establish automated high throughput screening, allowing rapid, directed design of purification protocols for therapeutic cells. The process is more rapid and reproducible than manual methods, enabling 96 samples to be processed in four hours. Volumes between 10 and 990 µl are pipetted using the Liquid Handling Arm with fixed tips, with standard deviations ranging from 1.5 % for the smallest volumes to less than 0.5 % for larger volumes, even with very viscous polymer solutions. We can also accurately pipette small cell suspension volumes, reproducibly transferring 5 x 105 cells in a volume of 13 µl with a standard deviation of less than 3 %. The automated process has been applied to the separation of differentiated and undifferentiated cells from the HL-60 cell line, achieving gentle, cost-effective and scalable purification of cell products without significantly influencing the cell viability.”

To find out more about Tecan’s cell biology solutions, visit www.tecan.com/cellbiology

 
For more information please contact

Tecan Trading AG
Antonietta Allocca
Seestrasse 103
CH-8708 Männedorf

Tel +41 (0)44 922 81 11
Fax +41 (0)44 922 81 12

info@tecan.com
www.tecan.com



About Tecan

Tecan (www.tecan.com) is a leading global provider of laboratory instruments and solutions in biopharmaceuticals, forensics and clinical diagnostics. The company specializes in the development, production and distribution of automated workflow solutions for laboratories in the life sciences sector. Its clients include pharmaceutical and biotechnology companies, university research departments, and forensic and diagnostic laboratories. As an original equipment manufacturer (OEM), Tecan is also a leader in developing and manufacturing OEM instruments and components that are then distributed by partner companies. Founded in Switzerland in 1980, the company has manufacturing, research and development sites in both Europe and North America and maintains a sales and service network in 52 countries. In 2014, Tecan generated sales of CHF 400 million (USD 437 million; EUR 331 million). Registered shares of Tecan Group are traded on the SIX Swiss Exchange (TECN; ISIN CH0012100191).