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Understanding imaging cell counting solutions

By Siegfried Sasshofer

All researchers performing cellular assays – research or clinical - need a cell counting solution. Cell counters are used to count cells in a culture to determine density, concentration or viability. Having established the need to count cells, how then to understand the many cell counting technologies available? Manual or automatic? Non-imaging (electrical resistance, flow, spectrophotometry) or imaging?

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When assessing confluence for cell-based assays, variety is not the spice of life

By Siegfried Sasshofer

Imagine life science research without cell-based assays. Or without cultured cells of all types to power those assays. Healthy, high-quality cells at the right point of confluence are vital for proliferation, kinetics, cytotoxicity, and gene expression studies particularly during long-term experiments. With so many different cell types, assay formats, and detection methods the variability inherent in cell-based assays can be enormous. There’s no room for inconsistency in cell counts and confluence assessments — it’s counterproductive and just wastes time. What’s the best way to improve counting accuracy in your cell-based assays?

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Cell counting by the numbers

By Michael Fejtl

“When you can measure what you are speaking about, and express it in numbers, you know something about it.” Lord Kelvin knew that. To be confident in your results, to quickly move your studies forward, and to be the first to publish your conclusions, you need to know that your numbers are right. The proof you need lies in reproducibility, and reproducibility in any cell-based assay starts with accurate cell counts.

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