Live cell imaging is one of the most important techniques in the life sciences today. But behind every great imaging assay, pity the poor scientist grappling with the demands of biological variability and complex kinetic cell assays. Live cell experiments are often synonymous with unsociable working hours, tedious protocols and unrepeatable results. In this blog we explore what it takes to tame automated cell imaging assays and take back control of kinetic experiments to get reliable results more quickly, with fewer errors, and less aggravation.
Cell-based assays are giving us deeper insight into cellular mechanisms in a true biological context, and fluorescence assays are playing a leading role. Applications range from cytotoxicity, proliferation, apoptosis and G-protein-coupled receptor (GPCR) signaling assays to high-throughput screening (HTS) drug discovery.
Imagine life science research without cell-based assays. Or without cultured cells of all types to power those assays. Healthy, high-quality cells at the right point of confluence are vital for proliferation, kinetics, cytotoxicity, and gene expression studies particularly during long-term experiments. With so many different cell types, assay formats, and detection methods the variability inherent in cell-based assays can be enormous. There’s no room for inconsistency in cell counts and confluence assessments — it’s counterproductive and just wastes time. What’s the best way to improve counting accuracy in your cell-based assays?
As we have seen in the previous posts in this series, implementing fluorescence detection will be a quick and effective route to improving the quality and sensitivity of your assays. Achieving optimal fluorescence assays requires an optics system with both sensitivity and flexibility.
Fluorescence detection can give you the ability to develop assays with extreme sensitivity, high robustness and a broad dynamic range. Success involves addressing several challenges, such as the careful choice of excitation (Ex) and emission (Em) wavelengths and the selection of flexible and sensitive optics, as we will see here.
Compared to many other detection technologies, fluorescence provides hard-to-beat performance and flexibility. Fluorescent labels are stable for months, deliver high sensitivity and the diversity in available dyes gives nearly unlimited possibilities in assay design. This and many other advantages make implementing fluorescence detection one of the easiest and safest ways for you to improve the quality and sensitivity of your assays.