Selected category: nucleic acid purification

By Beatrice Marg-Haufe

One of the steps in DNA sample preparation that is often overlooked when moving from manual to automated methods, is the quantification and normalization of nucleic acid samples that are destined for downstream analysis in different techniques and applications such as genotyping and NGS.

By Beatrice Marg-Haufe

Written in collaboration with Zymo Research, Irvine, CA, USA.

Applications based on next-generation sequencing (NGS), and more recently third-generation sequencing, play a central and ever-growing role in disease research.  There is a concurrent need for reliable, high-throughput nucleic acid purification  systems to feed samples into the analysis workflow for these applications. To meet this need, laboratories must either invest in more manpower to process samples manually, or transition to a liquid handling platform that can automate the workflow in a manner appropriate for the downstream application.