Cell culture is at the heart of the production process for many biopharmaceuticals, but finding the optimal conditions to maximize yield can be a complex and time-consuming process. Traditional process development relies on costly and labor-intensive set-ups, significantly limiting throughput and the range of experimental conditions that can be assessed. Scientists in Roche Pharma Research and Early Development (pRED) have adopted an alternative approach, combining single-use microbioreactors with advanced automation and analytical platforms to streamline the workflow.
The initial screening of chromatographic conditions can be a major bottleneck in the development of protein purification protocols. Italian company BiCT – Biological and Chemical Technologies – has turned to automation to overcome this issue, implementing parallel processing of miniature chromatography columns on a liquid handling platform to enable rapid simultaneous screening of a range of conditions. This maximizes the prospect of achieving the best result for each project while saving time and resources.
NGNY Devices is striving to fully automate laborious pre-analytical sample processing for clinical testing, helping to increase throughput and reduce human error. Its systems are already providing advanced sample sorting and aliquoting across Europe, offering a significant advantage over manual sample handling for busy clinical labs.
Our automation laboratory serves principally the lead optimization stage of drug discovery, so we receive several hundred samples per week from medicinal chemistry labs,” said Dr Claude Dufresne, Senior Investigator, who has been working at the Merck site since 1988 and has been developing automation methods and systems for most of the past ten years. “From those samples, we prepare serial dilution plates for distribution to various biological assay laboratories and for our own uses. We also store the samples in order to supply biology labs with samples for retesting and secondary assays; that’s why we needed the SSS
The Necker Hospital for Sick Children has automated its comparative genomic hybridization workflow on a Freedom EVO® workstation, increasing throughput more than 10-fold while significantly improving reproducibility and process security.
Scientists working in the Screening Unit at the Institute for Molecular Pharmacology in Germany are finding the low volume pipetting capability of the MultiChannel Arm™ 384 beneficial for the investigation of siRNAs and small molecule compound screening.
GlaxoSmithKline’s quest for improved anti-malarial treatments has been revolutionized by the speed and reliability of the HP D300 Digital Dispenser. This innovative system has increased throughput for standard drug titrations and enabled combinations of candidate compounds to be tested much more easily.
Symrise is using an HP D300 Digital Dispenser to improve throughput and reproducibility in its cell and molecular biology laboratories, offering more reproducible results for cosmetic active ingredient testing.
The ARUP Institute for Clinical and Experimental PathologyR (ARUP), based in Salt Lake City, USA, has chosen the Freedom EVOR liquid handling platform for automating RIA and ELISA assays, developing a novel 104-well microplate to increase sample throughput.
A successful partnership between Tecan and the Hochschule für Technik in Rapperswil, Switzerland, has resulted in the development of a new technology called Array Liquid Arrival Check (ArrayLAC) that shows great potential for built-in process control in Tecan pipetting platforms. ArrayLAC will be ideal for real time and parallel volume measurement in microplates before and after pipetting steps.
Cellular Dyncamics in Wisconsin, USA, is using its Celleritiy™ workstation for reliable culturing and differentiation of pluripotent steem cells, helping to provide the pharmaceutical industry with advanced tools for drug discovery and toxicology testing.
The Justus-von-Liebig soil sampling laboratory in Bavaria has what is probably the oldest suite of Tecan instruments still in routine use - unless you can tell us otherwise! - and has been using it to test agricultural soil samples for almost twenty years
MedImmune in Maryland, USA, is using its Freedom EVO® platforms as general-purpose tools for simple and routine tasks, rather than specific projects. This 'tools' concept has greatly increased use of the systems, saving much development time.
The Clinical Pharmacology Department in the Mahidol-Oxford Tropical Medicine Research Unit (MORU), Bangkok, has achieved huge time saving with its Freedom EVO® platform from Tecan, reducing time taken for sample preparation from five hours manually to just 20 minutes.
The Korea Center for Disease Control and Prevention (KCDC) in Seoul, Korea, conducts research to identify the etiology of communicable diseases and to find effective control, prevention, diagnosis and treatment for them.
The High Throughput Expression Laboratory at the University of York is a service facility offering bespoke protein expression, and uses a Freedom EVOR 200 workstation to meet its demand for high throughput cloning, expression and screening.
Advanced Technologies Cambridge (ATC), a plant biotechnology company located on the Cambridge Science Park, UK, has chosen the Tecan Freedom EVO® 100 liquid handling workstation to perform complex pipetting tasks for setting up PCR screening assays.
Illumina, a San Diego (CA)-based company, has integrated Tecan liquid handling workstations into its revolutionary, endto- end solutions for large-scale genotyping applications. The collaboration has been crucial for the development of a number of products, including Illumina’s production-scale BeadLab systems, which were deployed by major investigators world wide as part of the International HapMap Project and, more recently, Illumina’s bench-top BeadStation.